Biomimetic ABO blood-group typing
Biomimetic ABO blood-group typing
复制标题
DOI：
10.1002/anie.200502857
复制
发表时间：
2006-01-01
影响因子：
16.6
通讯作者：
Dickert, FL
中科院分区：
文献类型：
--
作者：
Hayden, O;Mann, KJ;Dickert, FL
血型A、B、AB和O通常通过使样品通过含有固定化抗体的葡聚糖凝胶，然后离心来确定。如果血型与抗体相对应，则形成沉淀物。[8]创新的自动化系统可以通过激光束反射率的变化来监测表面上的这种反应。然而，我们提出的MIPs功能可逆，并且不需要昂贵的试剂。我们的表面压印概念图1所示是基于软光刻合成的MIP直接在传感器上涂有聚合薄膜。这种紧密堆积的识别位点的合理制造与潜在的大规模生产的技术过程相容，并且不需要昂贵的抗体。在显微镜载玻片上制备覆盖有单层红细胞的印章（图2a）。模塑聚氨酯表面上的空腔对应于红细胞的环形形貌（图2b，c）。细胞形成深度高达500 nm的印记（图2d）。参比电极上的非压印层（通过旋涂产生）是光滑的（参见支持信息）。生物印迹进行微天平与两个电极的差分测量;一个电极被用作非印迹参考，以消除粘度的影响。[9]我们利用双重结构的优势，在相同的条件下观察红细胞在印迹和非印迹聚氨酯上的选择性和非选择性吸附，红细胞悬浮液在印迹或非印迹A型血（BGA）红细胞的聚氨酯上的吸附如图3a所示。红细胞浓度比全血中的浓度（4-5 109个细胞/mLX-1）稀释两个数量级以上。印迹表面表现出选择性吸附的血型A的红细胞，而轻微的非选择性吸附观察到与非印迹聚氨酯。非选择性吸附从流动条件下的特定效果的约5%增加到当流动由于沉降而停止时的20%。B型红细胞的吸附量仅为A型血的三分之一，这表明红细胞具有血型选择性识别。值得注意的是，BGB电池的非选择性吸附与BGA相同。
The blood groups A, B, AB, and O are usually determined by passing a sample through a dextran gel containing immobilized antibodies followed by centrifugation. If the blood group corresponds to the antibody, a precipitate forms.[8] Innovative automated systems are available that monitor this reaction on a surface by changes in the reflectance of a laser beam. The MIPs we propose function reversibly, however, and no expensive reagents are necessary. Our surface-imprinting concept depicted in Figure 1 is based on a soft-lithographic synthesis of MIPs directly on a transducer coated with a polymerizing thin film. This rational fabrication of closely packed recognition sites is compatible with technological processes for potential large-scale production and does not require expensive antibodies. Stamps covered with monolayers of red blood cells were prepared on microscope slides (Figure 2a). The cavities on the molded polyurethane surface correspond to the doughnut-shaped topography of the erythrocytes (Figure 2b, c). The cells form imprints with depths up to 500 nm (Figure 2d). The nonimprinted layer on the reference electrode, which is produced by spin coating, is smooth (see Supporting Information). The bioimprinting was performed on microbalances with two electrodes for differential measurements; one electrode was used as a nonimprinted reference to eliminate viscosity effects.[9] We take advantage of the dual construction to observe selective and nonselective adsorption of red blood cells on imprinted and nonimprinted polyurethane under identical conditions.The adsorption of suspensions of red blood cells on polyurethane imprinted with or nonimprinted with blood groupA (BGA) erythrocytes is shown in Figure3a. The erythrocyte concentration is more than two orders of magnitude more dilute than that in whole blood (4–5 109 cells mLÀ1). Imprinted surfaces show selective adsorption of erythrocytes of blood group A, whereas minor nonselective adsorption is observed with nonimprinted polyurethane. The nonselective adsorption increases from about 5% of the specific effect under flow conditions to 20% when the flow has stopped because of sedimentation. Erythrocytes of type B show only one-third of the adsorption of blood groupA, indicating a blood-type-selective recognition. It is noteworthy that the nonselective adsorption of BGB cells is the same as that of BGA.