All three SOS-inducible DNA polymerases (Pol II, Pol IV and Pol V) are involved in induced mutagenesis
All three SOS-inducible DNA polymerases (Pol II, Pol IV and Pol V) are involved in induced mutagenesis
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DOI：
10.1093/emboj/19.22.6259
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发表时间：
2000-11-15
期刊：
影响因子：
11.4
通讯作者：
Fuchs, RPP
中科院分区：
文献类型：
--
作者：
Napolitano, R;Janel-Bintz, R;Fuchs, RPP
大多数生物体含有最近发现的一类DNA聚合酶（umuC/dinB超家族）的几个成员，其可能参与受损DNA的复制。在大肠杆菌中，已知只有Pol V（umuDC）对于由UV光或无碱基位点诱导的碱基置换诱变是必需的。在这里，我们表明，根据DNA损伤的性质及其序列背景，另外两个SOS诱导的DNA聚合酶，Pol II（polB）和Pol IV（dinB），也参与了无错误和诱变translesion合成（TLS）。例如，位于NarI突变热点内的N-2-乙酰氨基芴（AAF）鸟嘌呤加合物的旁路需要Pol II用于-2移码，但Pol V用于无错误TLS。另一方面，苯并（a）芘加合物处的无错误和-1移码TLS需要Pol IV和Pol V两者。该细胞使用“跨损伤”DNA聚合酶池以绕过各种DNA损伤。
Most organisms contain several members of a recently discovered class of DNA polymerases (umuC/dinB superfamily) potentially involved in replication of damaged DNA, In Escherichia coli, only Pol V (umuDC) was known to be essential for base substitution mutagenesis induced by UV light or abasic sites. Here we show that, depending upon the nature of the DNA damage and its sequence context, the two additional SOS-inducible DNA polymerases, Pol II (polB) and Pol IV (dinB), are also involved in error-free and mutagenic translesion synthesis (TLS). For example, bypass of N-2-acetylaminofluorene (AAF) guanine adducts located within the NarI mutation hot spot requires Pol II for -2 frameshifts but Pol V for error-free TLS, On the other hand, error-free and -1 frameshift TLS at a benzo(a)pyrene adduct requires both Pol IV and Pol V, Therefore, in response to the vast diversity of existing DNA damage, the cell uses a pool of 'translesional' DNA polymerases in order to bypass the various DNA lesions.