Characterization of CD8+ T lymphocytes in chronic HIV-1 subtype A infection in Rwandan women
Characterization of CD8+ T lymphocytes in chronic HIV-1 subtype A infection in Rwandan women
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DOI：
10.1107/88
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发表时间：
2005-01-01
影响因子：
1.6
通讯作者：
Jolly, P. E.
中科院分区：
文献类型：
--
作者：
Jiang, Y.;Karita, E.;Jolly, P. E.
我们研究了卢旺达妇女的CD 8 + T淋巴细胞特征，这些妇女在没有抗逆转录病毒治疗的情况下存活了8-13年的HIV-1亚型A感染。在7名妇女中的5名的PBMC中可检测到表达HIV-1亚型A gag特异性IFN-γ的CD 8 + T淋巴细胞，并且这些细胞与血浆病毒载量之间存在强正相关（r = 0.9262; p < 0.001）。这些HIV阳性女性的CD 8 + T细胞比HIV阴性供体的细胞产生更多的RANTES和MIP-1 β。然而，与HIV阴性对照组（42.86 +/- 11.0%; p < 0.001）相比，感染妇女的CD 8 + T淋巴细胞产生的穿孔素水平极低（0.88 +/- 0.93%）。HIV感染者外周血中CD 8 + CD 45 RA+穿孔素+和CD 8 + CD 45 RA + CD 27-淋巴细胞的比例也明显低于HIV阴性对照组。因此，CD 8 + T细胞无法控制HIV可能部分取决于HIV特异性CD 8 + T细胞产生的效应物不足。此外，CD 8 + T细胞中穿孔素的缺乏与持续的CD 27表达有关，表明慢性HIV-1感染中的成熟受损和细胞溶解活性受损。
We examined CD8+ T lymphocyte characteristics in Rwandan women who have survived HIV-1 subtype A infection for 8-13 years without antiretroviral therapy. HIV-1 subtype A gag-specific IFN-gamma expressing CD8+ T lymphocytes were detectable in PBMC from 5 of 7 women and there was a strong positive association (r = 0.9262; p < 0.001) between these cells and plasma viral load. CD8+ T cells of these HIV-positive women produced more RANTES and MIP-1beta than cells from HIV-negative donors. However, extremely low levels of perforin (0.88 +/- 0.93%) were produced by CD8+ T lymphocytes from the infected women compared with HIV-negative controls (42.86 +/- 11.0%; p < 0.001). The percentages of CD8+CD45RA+perforin+ and CD8+CD45RA+CD27-lymphocytes were also much lower in the HIV-infected women compared with HIV-negative controls. Therefore, the inability of CD8+ T cells to control HIV may, in part, be dependent on insufficient generation of effectors from HIV-specific CD8+ T cells. Also, the lack of perforin in CD8+ T cells is linked with persistent CD27 expression, suggesting impaired maturation and impairment in cytolytic activity in chronic HIV-1 infection.