Therapeutic effect of quercetin in collagen-induced arthritis
Therapeutic effect of quercetin in collagen-induced arthritis
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DOI：
10.1016/j.biopha.2017.03.026
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发表时间：
2017-06-01
影响因子：
7.5
通讯作者：
Ketheesan, Natkunam
中科院分区：
文献类型：
--
作者：
Haleagrahara, Nagaraja;Miranda-Hernandez, Socorro;Ketheesan, Natkunam
槲皮素是一种生物活性黄酮类化合物，具有抗炎、免疫抑制和保护特性，是治疗类风湿性关节炎 (RA) 的潜在药物。胶原诱导的关节炎（CIA）是研究 RA 发病机制最常用的动物模型。本研究分析了槲皮素对 C57BL/6 小鼠胶原诱导的关节炎的治疗作用。将动物分为五组，接受以下治疗：阴性（未治疗）对照、阳性对照（关节炎诱导）、关节炎+甲氨蝶呤、关节炎+槲皮素和关节炎+甲氨蝶呤+槲皮素。对体重、水肿、关节损伤和细胞因子产生的评估用于确定槲皮素的治疗效果。这项研究首次证明了槲皮素在CIA体内的抗炎和保护作用。结果还表明，槲皮素和甲氨蝶呤的同时给药并没有比单一药物的给药提供更大的保护。使用槲皮素作为单一治疗剂可实现最低程度的关节炎症和最高的保护作用。使用槲皮素治疗的动物中疾病严重程度的减轻与 TNF-α、IL-1β、IL-17 和 MCP-1 水平的降低有关。总之，这项研究确定无毒的槲皮素在保护小鼠关节免受关节炎炎症方面比甲氨蝶呤产生更好的效果。槲皮素可能是 RA 的替代治疗方法，因为它可以调节 RA 的主要致病途径。 (C) 2017 Elsevier Masson SAS。版权所有。
Quercetin, a bioactive flavonoid with anti-inflammatory, immunosuppressive, and protective properties, is a potential agent for the treatment of rheumatoid arthritis (RA). Collagen-induced arthritis (CIA) is the most commonly used animal model for studying the pathogenesis of RA. This study analysed the therapeutic role of quercetin in collagen-induced arthritis in C57BL/6 mice. The animals were allocated into five groups that were subjected to the following treatments: negative (untreated) control, positive control (arthritis-induced), arthritis + methotrexate, arthritis + quercetin, and arthritis + methotrexate + quercetin. Assessments of weight, oedema, joint damage, and cytokine production were used to determine the therapeutic effect of quercetin. This study demonstrated for the first time the anti-inflammatory and protective effects of quercetin in vivo in CIA. The results also showed that the concurrent administration of quercetin and methotrexate did not offer greater protection than the administration of a single agent. The use of quercetin as a monotherapeutic agent resulted in the lowest degree of joint inflammation and the highest protection. The reduced severity of the disease in animals treated with quercetin was associated with decreased levels of TNF-alpha, IL-1 beta, IL-17, and MCP-1. In conclusion, this study determined that quercetin, which was non-toxic, produced better results than methotrexate for the protection of joints from arthritic inflammation in mice. Quercetin may be an alternative treatment for RA because it modulates the main pathogenic pathways of RA. (C) 2017 Elsevier Masson SAS. All rights reserved.