Effect of sulfated polysaccharides from Laminaria japonica on vascular endothelial cells in psychological stress rats.
Effect of sulfated polysaccharides from Laminaria japonica on vascular endothelial cells in psychological stress rats.
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DOI：
10.1016/j.jep.2013.11.014
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发表时间：
2014-01
影响因子：
5.4
通讯作者：
Jing Li;Sheng-Nan Wang;Xiao-mei Yang;Guangbao Pang;Hua Zheng;Bingxiang Shen;Guanhong Li;D. Shi;Jienian Wang;Liaoyun Feng;Mu-Yan Li;Wu Wei;Wu Qin;Lu Xie
中科院分区：
文献类型：
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作者：
Jing Li;Sheng-Nan Wang;Xiao-mei Yang;Guangbao Pang;Hua Zheng;Bingxiang Shen;Guanhong Li;D. Shi;Jienian Wang;Liaoyun Feng;Mu-Yan Li;Wu Wei;Wu Qin;Lu Xie
民族药理学相关性海带是中国常见的海鲜和药用植物。海带在中药中用于治疗和预防高血压和水肿。材料与方法研究了硫酸化多糖对心理应激（PS）后肾上腺素诱导的大鼠血管内皮损伤的血管保护活性和机制。载体（假手术组和 PS 组）、海带硫酸多糖（LP；1 mg/kg 和 5 mg/kg）和依诺肝素钠（1 IU/kg，参考药物）均给药 10 天。记录行为变化。测量肾上腺素、皮质醇、单胺氧化酶（MAO）、氨基脲敏感胺氧化酶（SSAO）、甲醛、H2O2、一氧化氮（NO）、内皮素-1（ET-1）、6-酮-前列腺素F1a（6-酮-PGF1a）和血栓素B2（TXB2）的血浆水平。测量胸主动脉内皮依赖性舒张，并对主动脉血管进行透射电镜观察。结果LP组大鼠血浆中肾上腺素代谢物明显低于PS组（P<0.01）。 LP治疗后血浆NO/ET-1和6-酮-PGF1a/TXB2的正常比值得以维持，主动脉内皮依赖性舒张作用大大增强（P<0.05）。与假手术组相比，PS 大鼠血管内皮细胞 (VEC) 发生形态学改变，溶酶体数量增多，线粒体嵴模糊。然而，LP治疗后这些组织病理学变化明显减轻。结论本研究显示LP对PS大鼠VECs具有保护作用。 LP可调节血浆NO、ET-1和6-keto-PGF1a水平，增强内皮依赖性舒张，减轻VEC中溶酶体和线粒体的组织病理学变化。 LP对PS大鼠VECs的潜在作用机制与其减少肾上腺素代谢物的功能有关。
Ethnopharmacological relevanceLaminaria japonicais a popular seafood and medicinal plant in China.Laminaria japonicais used in traditional Chinese medicine to treat and prevent hypertension and edema.Materials and methodsThe vascular protective activity and mechanism of sulfated polysaccharides were studied in adrenalin-induced vascular endothelial damage in rats after psychological stress (PS). Vehicle (sham and PS groups), sulfated polysaccharide fromLaminaria japonica(LP; 1 mg/kg and 5 mg/kg) and enoxaparin sodium (1 IU/kg, reference drug) were all administered for 10 days. Behavioral changes were recorded. Plasma levels of adrenalin, cortisol, monoamine oxidase (MAO), semicarbazide-sensitive amine oxidase (SSAO), formaldehyde, H2O2, nitric oxide (NO), endothelin-1 (ET-1), 6-keto-prostaglandin F1a(6-keto-PGF1a), and thromboxane B2(TXB2) were measured. Endothelium-dependent relaxation of the thoracic aorta was measured and transmission electron microscopy of aortic vessels was performed.ResultsAdrenalin metabolites in plasma were significantly lower (P<0.01) in rats after LP administration compared with those in the PS groups. The normalized ratios of plasma NO/ET-1 and 6-keto-PGF1a/TXB2were maintained and endothelium-dependent relaxation of the aorta was greatly enhanced after LP treatment (P<0.05). Morphological alterations were observed in vascular endothelial cells (VECs) in PS rats, with a higher number of lysosomes and vague mitochondrial cristae compared with those in the sham group. However, these histopathological changes were markedly alleviated after LP treatment.ConclusionsThis study shows a protective effect of LP on VECs in PS rats. LP can regulate plasma levels of NO, ET-1, and 6-keto-PGF1a, enhance endothelium-dependent relaxation, and alleviate histopathological changes of lysosomes and mitochondria in VECs. The potential mechanism of LP on VECs in PS rats is related to its function of reducing metabolites of adrenalin.