Specific receptor for endothelin in cultured rat cardiocytes.
Specific receptor for endothelin in cultured rat cardiocytes.
复制标题
培养的大鼠心肌细胞中内皮素的特异性受体。
DOI：
10.1016/s0006-291x(89)80165-2
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
F. Marumo
中科院分区：
文献类型：
--
作者：
Y. Hirata;Y. Fukuda;H. Yoshimi;T. Emori;M. Shichiri;F. Marumo
Specific binding sites for the endothelium-derived vasoconstrictor endothelin (ET) and its effect on cytosolic free Ca2+concentrations ([Ca2+]i) were studied in a primary culture of cardiocytes from neonatal rats. Binding studies using125I-labeled-porcine ET as a radioligand revealed the presence of a single class of high-affinity binding sites for ET in cardiocytes with an apparent Kdof 6−9×10−10M and a Bmaxof 50,000–80,000 sites/cell. Neither various vasoconstrictors nor Ca2+-channel blockers affected the binding. Pretreatment with ET substantially reduced the total number of ET receptors without changing their affinity. ET dose- dependently increased [Ca2+]iin fura-2-loaded cardiocytes. These data indicate that cardiocytes have specific ET receptors that are controlled by a down-regulation mechanism, and that ET induces a receptor-mediated increase in [Ca2+]iin cardiocytes.
