The effect of Plasmodium falciparum infection on expression of monocyte surface molecules
The effect of Plasmodium falciparum infection on expression of monocyte surface molecules
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DOI：
10.1016/j.trstmh.2006.01.005
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发表时间：
2006-11-01
影响因子：
2.2
通讯作者：
Craig, A. G.
中科院分区：
文献类型：
--
作者：
Jenkins, N. E.;Chakravorty, S. J.;Craig, A. G.
恶性疟原虫感染可能会导致易感人群患上严重的疟疾。严重疾病的发病机制可能是受感染红细胞的隔离和免疫反应过度刺激的结合。单核细胞爱尔兰是许多相关促炎剂的关键来源，但也被发现隔离在血管中。然而，人们对疟疾疾病中的单核细胞表型知之甚少。对新鲜全血进行流式细胞术，以确定急性重症和非重症疟疾期间以及未感染的恢复期期间四种受体的表面表达。研究人员对 356 名恶性疟原虫感染儿童进行了研究，结果发现，与恢复期相比，急性病期间细胞间粘附分子 1 (ICAM-1)、尿激酶纤溶酶原激活剂受体 (uPAR)、CD23 和趋化因子受体 5 (CCR5) 的表达增加（P < 0.001）。通过多变量分析，发现ICAM-1（比值比（OR）2.44，95% CI 1.80-3.32）和uPAR（OR 3.14，95% CI 1.93-5.09）表达大幅增加，但CD23表达小幅增加（OR 0.82，95% CI 0.68-0.96）与严重疟疾独立相关。这些结果让我们深入了解严重疟疾中发生的细胞过程，并表明病理学是基于促炎和抗炎过程的复杂过程。 (C) 2006 年由 Elsevier Ltd 代表英国皇家热带医学与卫生学会出版。
Plasmodium falciparum infection may result in severe malaria in susceptible individuals. The pathogenesis of severe disease is probably a combination of the sequestration of infected erythrocytes and overstimulation of the immune response. Monocytes Eire a key source of many of the pro-inflammatory agents implicated but also are found sequestered in blood vessels. However, little is known about the monocyte phenotype in malaria disease. Flow cytometry was performed on fresh whole blood to determine surface expression of four receptors during acute severe and non-severe malaria and again during convalescence when uninfected. Three hundred and fifty-six children with P falciparum infection were studied and were found to show increased expression of intercellular adhesion molecule-1 (ICAM-1), urokinase plasminogen activator receptor (uPAR), CD23 and chemokine receptor 5 (CCR5) (P < 0.001) during acute disease compared with convalescent levels. Using multivariate analysis, it was found that large increases in expression of ICAM-1 (odds ratio (OR) 2.44, 95% CI 1.80-3.32) and uPAR (OR 3.14, 95% CI 1.93-5.09) but small increases in expression of CD23 (OR 0.82, 95% CI 0.68-0.96) were independently associated with severe malaria. These results give an insight into the cellular processes occurring in severe malaria and suggest that pathology is based on a complex repertoire of pro- and anti-inflammatory processes. (C) 2006 Published by Elsevier Ltd on behalf of Royal Society of Tropical Medicine and Hygiene.