Proteomic differences with and without ozone-exposure in a smoking-induced emphysema lung model.
Proteomic differences with and without ozone-exposure in a smoking-induced emphysema lung model.
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DOI：
10.3904/kjim.2015.30.1.62
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发表时间：
2015-01
期刊：
影响因子：
--
通讯作者：
Park CS
中科院分区：
文献类型：
--
作者：
Uh ST;Koo SM;Jang AS;Park SW;Choi JS;Kim YH;Park CS
慢性阻塞性肺疾病的急性加重可能与空气污染有关，臭氧是空气污染的重要组成部分。在这项研究中，我们在吸烟诱导的肺气肿模型中研究了与臭氧诱导的恶化相关的蛋白质谱。将小鼠分为不吸烟不臭氧(NS+NO)组、不吸烟不臭氧(NS+O)组、吸烟不臭氧(S+NO)组和吸烟臭氧(S+O)组。采用支气管肺泡灌洗、苏木精伊红染色的平均线性截距(MLI)、纳米液质联质谱(LC-MS/MS)和Western blotting分析。Ⅲ组(S+NO)和IV组(S+O)的MLI值分别为45±2和44±3µm，显著高于I组(NS+NO)和II组(NS+O组)(分别为26±2和23±2µm；P<0.05)。用LC-MS/MS鉴定了I组(NS+NO)蛋白质双向电泳图像分析中有14个蛋白质强度差异显著的斑点，其中IV组(S+O组)6个蛋白质水平较高。Western blotting和蛋白质组学分析显示，吸烟和臭氧处理均降低了波形蛋白、乳酸脱氢酶A和磷酸三糖异构酶的水平。相反，吸烟和臭氧处理均增加了Tbc1结构域家族5(TBC1D5)和层蛋白A的表达。TBC1D5可作为臭氧致肺气肿肺损伤的生物标志物。
Acute exacerbations in chronic obstructive pulmonary disease may be related to air pollution, of which ozone is an important constituent. In this study, we investigated the protein profiles associated with ozone-induced exacerbations in a smoking-induced emphysema model. Mice were divided into the following groups: group I, no smoking and no ozone (NS + NO); group II, no smoking and ozone (NS + O); group III, smoking and no ozone (S + NO); and group IV, smoking and ozone (S + O). Bronchoalveolar lavage, the mean linear intercept (MLI) on hematoxylin and eosin staining, nano-liquid chromatography-tandem mass spectrometry (LC-MS/MS), and Western blotting analyses were performed. The MLIs of groups III (S + NO) and IV (S + O) (45 ± 2 and 44 ± 3 µm, respectively) were significantly higher than those of groups I (NS + NO) and II (NS + O) (26 ± 2 and 23 ± 2 µm, respectively; p < 0.05). Fourteen spots that showed significantly different intensities on image analyses of two-dimensional (2D) protein electrophoresis in group I (NS + NO) were identified by LC-MS/MS. The levels of six proteins were higher in group IV (S + O). The levels of vimentin, lactate dehydrogenase A, and triose phosphate isomerase were decreased by both smoking and ozone treatment in Western blotting and proteomic analyses. In contrast, TBC1 domain family 5 (TBC1D5) and lamin A were increased by both smoking and ozone treatment. TBC1D5 could be a biomarker of ozone-induced lung injury in emphysema.
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影响因子：
5.8
作者：
Manoli SE;Smith LA;Vyhlidal CA;An CH;Porrata Y;Cardoso WV;Baron RM;Haley KJ
通讯作者：
Haley KJ
影响因子：
4.3
作者：
Gerritsen, WBM;Asin, J;Haas, FJLM
通讯作者：
Haas, FJLM
在小鼠模型中锻炼肺肺气肿的耐受性和全身表现。
影响因子：
5.8
作者：
Lüthje L;Raupach T;Michels H;Unsöld B;Hasenfuss G;Kögler H;Andreas S
通讯作者：
Andreas S
影响因子：
4
作者：
Seaman, Matthew N. J.;Harbour, Michael E.;Bright, Nicholas
通讯作者：
Bright, Nicholas
DOI：
10.1111/j.1440-1681.2010.05411.x
发表时间：
2010-10-01
影响因子：
2.9
作者：
Liu, Zi-Bing;Song, Na-Na;Shen, Lin-Lin
通讯作者：
Shen, Lin-Lin