Quantitative Neuropeptidome Analysis Reveals Neuropeptides Are Correlated with Social Behavior Regulation of the Honeybee Workers
Quantitative Neuropeptidome Analysis Reveals Neuropeptides Are Correlated with Social Behavior Regulation of the Honeybee Workers
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定量神经肽组分析揭示神经肽与工蜂的社会行为调节相关
DOI：
10.1021/acs.jproteome.5b00632
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发表时间：
2015-10-01
影响因子：
4.4
通讯作者：
Li, Jianke
中科院分区：
文献类型：
--
作者：
Han, Bin;Fang, Yu;Li, Jianke
神经肽在协调生物体的神经通讯和生理调节方面发挥着至关重要的作用，充当神经递质、神经调节剂和神经激素。蜜蜂高度进化的社会结构是理解神经肽如何调节社会行为的良好系统；然而，关于与年龄相关的劳动分工中神经肽组学变异的许多知识仍然未知。对两种蜜蜂品系——意大利蜜蜂（Apis mellifera ligustica，ITb）和高产蜂王浆的蜜蜂（RJb，选择用于近四十年来提高中国 ITb 的蜂王浆产量）对与年龄相关的多民族性的四个时间点的大脑神经肽组动力学进行了深入比较。在 158 个已鉴定的非冗余神经肽中，有 77 个是之前未报道的，显着扩大了蜜蜂神经肽组的覆盖范围。事实上，ITb 和 RJb 中 14 个相同的神经肽前体在分工过程中表达水平发生了变化，这表明它们与蜜蜂工蜂的任务转换高度相关。这些观察结果进一步表明，这两个蜜蜂品系采用了类似的神经肽组修饰，通过调节排泄系统、生物钟系统等来调整各自的年龄多民族生理学。值得注意的是，RJb 中与调节水稳态、育雏信息素识别、觅食能力和花粉收集有关的神经肽水平的提高表明神经肽也参与了 RJ 分泌的调节。这些发现对与社会行为调节相关的蜜蜂神经肽组有了新的认识，这在蜜蜂和其他昆虫的神经生物学中具有潜在的重要意义。
Neuropeptides play vital roles in orchestrating neural communication and physiological modulation in organisms, acting as neurotransmitters, neuromodulators, and neurohormones. The highly evolved social structure of honeybees is a good system for understanding how neuropeptides regulate social behaviors; however, much knowledge on neuropeptidomic variation in the age-related division of labor remains unknown. An in-depth comparison of the brain neuropeptidomic dynamics over four time points of age-related polyethism was performed on two strains of honeybees, the Italian bee (Apis mellifera ligustica, ITb) and the high royal jelly producing bee (RJb, selected for increasing royal jelly production for almost four decades from the ITb in China). Among the 158 identified nonredundant neuropeptides, 77 were previously unreported, significantly expanding the coverage of the honeybee neuropeptidome. The fact that 14 identical neuropeptide precursors changed their expression levels during the division of labor in both the ITb and RJb indicates they are highly related to task transition of honeybee workers. These observations further suggest the two lines of bees employ a similar neuropeptidome modification to tune their respective physiology of age polyethism via regulating excretory system, circadian clock system, and so forth. Noticeably, the enhanced level of neuropeptides implicated in regulating water homeostasis, brood pheromone recognition, foraging capacity, and pollen collection in RJb signify the fact that neuropeptides are also involved in the regulation of RJ secretion. These findings gain novel understanding of honeybee neuropeptidome correlated with social behavior regulation, which is potentially important in neurobiology for honeybees and other insects.