Expression of long pentraxin PTX3 in human adipose tissue and its relation with cardiovascular risk factors
Expression of long pentraxin PTX3 in human adipose tissue and its relation with cardiovascular risk factors
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DOI：
10.1016/j.atherosclerosis.2008.05.015
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发表时间：
2009-02-01
期刊：
影响因子：
5.3
通讯作者：
Invitti, C.
中科院分区：
文献类型：
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作者：
Alberti, L.;Gilardini, L.;Invitti, C.
正五聚蛋白3（PTX 3）是一种急性时相蛋白，在晚期动脉粥样硬化病变中强烈表达。我们研究了（a）从21名肥胖者中获得的皮下脂肪组织（SAT）和网膜内脏脂肪组织（VAT）中PTX 3的表达和分泌，（37.4 ± 8.15岁）和10例正常体重受试者（43.7 +/- 11.07岁）和（B）脂肪PTX 3与肿瘤坏死因子α的关系（TNF α）和脂联素表达与心脏代谢危险因素。实时PCR用于定量PTX 3、CD 68（巨噬细胞标志物）、TNF α和脂联素的特异性mRNA。培养新鲜脂肪组织并在培养基中测量PTX 3。测定血清胰岛素、血糖、高密度脂蛋白和低密度脂蛋白胆固醇、甘油三酯、C反应蛋白（CRP）、纤维蛋白原、脂联素、TNF α和PTX 3。PTX 3表达在两个脂肪隔室中是相似的，并且仅在VAT中，肥胖受试者的PTX 3表达倾向于高于正常体重受试者（p=0.05）。CD 68和PTX 3的表达与仙人掌有关，而在SAT中则无相关性。校正年龄和性别后，VAT-PTX 3表达和释放与VAT-TNF α表达（两者p < 0.01）和LDL/HDL比值（p < 0.01和p < 0.001）相关。VAT PTX 3表达与BMI、甘油三酯、CRP、纤维蛋白原和脂联素相关（p
Pentraxin 3 (PTX3) is an acute phase protein strongly expressed by advanced atherosclerotic lesions. We investigated (a) PTX3 expression and secretion in subcutaneous adipose tissue (SAT) and omental visceral adipose tissue (VAT) obtained from 21 obese (37.4 +/- 8.15 yr) and 10 normal weight subjects (43.7 +/- 11.07 yr) and (b) the relationships of adipose PTX3 with tumour necrosis factor alpha (TNF alpha) and adiponectin expression and with cardiometabolic risk factors. Real-time PCR was used to quantify specific mRNA for PTX3, CD68 (macrophage marker), TNF alpha and adiponectin. Fresh adipose tissue was cultured and PTX3 measured in the medium. Serum insulin, glucose, HDL and LDL cholesterol, triglycerides, C-reactive protein (CRP), fibrinogen, adiponectin, TNF alpha and PTX3 were measured. PTX3 expression was similar in the two fat compartments and tended to be higher in obese than in normal weight subjects in VAT only (p=0.05). CD68 and PTX3 expressions were correlated with cacti other in SAT but not in VAT. After adjustment for age and sex, VAT PTX3 expression and release were correlated with VAT-TNF alpha expression (p < 0.01 for both) and with LDL/HDL ratio (p < 0.01 and p < 0.001). VAT PTX3 expression was also correlated with BMI, triglycerides, CRP, fibrinogen and adiponectin (p