Morphogenesis of avian infectious bronchitis virus in primary chick kidney cells.
Morphogenesis of avian infectious bronchitis virus in primary chick kidney cells.
复制标题
DOI：
10.1007/bf01317869
复制
发表时间：
1976
影响因子：
2.7
通讯作者：
Alexander DJ
中科院分区：
文献类型：
--
作者：
Chasey D;Alexander DJ
用鸡传染性支气管炎病毒（IBV）感染原代鸡肾细胞，进行电镜观察。病毒颗粒进入细胞的viropexis和细胞过程（吞噬作用）和微胞饮进入包被运输囊泡之间的区别。病毒成熟发生的出芽成池的内质网或细胞质空泡，并获得证据表明，病毒表面的预测可以在出芽过程中连接。在感染后期，大量的病毒颗粒存在，主要在细胞质空泡中，并且大多数通过细胞溶解而释放。还观察到空泡与质膜融合释放，单个病毒粒子可从内质网运输到包被囊泡内的表面。
Primary chick kidney cells were infected with avian infectious bronchitis virus (IBV) and examined by electron microscopy. Virus particles entered the cells by viropexis and distinction could be made between engulfment by cell processes (phagocytosis) and entry by micropinocytosis in coated transport vesicles. Virus maturation occurred by budding into either the cisternae of the endoplasmic reticulum or cytoplasmic vacuoles, and evidence was obtained to suggest that the viral surface projections could be attached during the budding process. Late in infection large numbers of virus particles were present, mainly in cytoplasmic vacuoles, and the majority were released by cell lysis. Release by fusion of vacuoles with the plasma membrane was also observed, and individual virions could be transported from the endoplasmic reticulum to the surface within coated vesicles.