Evaluation of techniques for detection of small trematode eggs in faeces of domestic animals
Evaluation of techniques for detection of small trematode eggs in faeces of domestic animals
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DOI：
10.1016/j.vetpar.2008.05.021
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发表时间：
2008-10-01
影响因子：
2.6
通讯作者：
Thamsborg, Stig Milan
中科院分区：
文献类型：
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作者：
Anh, Nguyen Thi Lan;Phuong, Nguyen Thi;Thamsborg, Stig Milan
本研究旨在评价犬、猫和猪粪便中小吸虫卵的检测技术。采用Kato-Katz技术（KK）、福尔马林-乙基沉降技术（FE）以及由丹麦卫生研究与发展中心（原丹麦血吸虫病实验室）（DBL）开发的过滤、沉降和离心相结合的方法对狗（80例）、猫（35例）和猪（114例）的粪便样本进行检测。对38只狗，25只猫和16头猪进行了尸检，被认为是评估技术的金标准方法。结果显示这三种技术的特异性为100%。与DBL-和fe -技术相比，kk技术在狗样本中的灵敏度较低。这三种技术对猫和猪的敏感性相似。基于这些发现和实际问题，选择dbl技术是最合适的，因为它易于检测和定量。与fe技术相比，不需要有毒化学品或特殊设备，fe技术需要乙醚溶液，因此需要通风柜，而越南和其他发展中国家的当地兽医中心往往没有这些设备。(C) 2008 Elsevier B.V.版权所有
This study was conducted to evaluate techniques for detection of small trematode eggs in faeces of dogs, cats and pigs. Faecal samples from dogs (n = 80), cats (n = 35) and pigs (n = 114) were examined by Kato-Katz technique (KK), formalin-ether sedimentation technique (FE) and a method of combining: filtration, sedimentation and centrifugation, developed at DBL - Centre for Health Research and Development (former Danish Bilharziasis Laboratory) (DBL). Necropsy was performed on 38 dogs, 25 cats and 16 pigs and was considered as a gold standard method for evaluation of the techniques. The results showed 100% specificity for the three techniques. Lower sensitivity was seen for the KK-technique in dog samples in comparison to that for DBL- and FE-technique. The sensitivity of the three techniques was similar in cats and pigs. Based on these findings and practical issues, DBL-technique was chosen as most suitable because the eggs were easily detected and quantified. No toxic chemicals or special equipment were required in comparison with FE-techniquc that needs ether solution and thus fume cupboards which are often unavailable in local veterinary centres in Vietnam and other developing countries. (C) 2008 Elsevier B.V. All rights reserved.