Gene sequencing and expression of Raf-1 in lymphatic metastasis of hypopharyngeal carcinoma
Gene sequencing and expression of Raf-1 in lymphatic metastasis of hypopharyngeal carcinoma
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DOI：
10.3233/cbm-191238
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发表时间：
2020-01-01
影响因子：
3.1
通讯作者：
Hu, Guohua
中科院分区：
文献类型：
--
作者：
Li, Yanshi;Lu, Tao;Hu, Guohua
目的：应用基因测序技术对下咽癌淋巴转移进行鉴别基因筛选。我们还旨在探讨筛选的基因在下咽癌淋巴转移中的表达及其临床病理意义。方法：收集98例下咽癌患者的临床病理特征，采用Kaplan-Meier和log-rank检验进行生存分析。对6例有或无淋巴转移患者的肿瘤组织进行差异表达基因的基因测序。在另外20例诊断为下咽癌的患者中，通过RT-PCR和Western blot验证了最常见的差异表达基因。采用Kaplan-Meier & log-rank检验和Cox’s检验，对70例下咽癌肿瘤组织和正常组织进行免疫组化表达检测，探讨其预后价值。结果：淋巴转移已被证明会导致下咽癌患者术后生存率降低。基因测序分析结果显示，Raf-1在下咽癌淋巴转移中是差异表达基因。此外，与非淋巴转移的肿瘤组织和正常组织相比，淋巴转移患者肿瘤组织中Raf-1的表达明显上调。同时，Raf-1已被证实是影响下咽癌预后的独立危险因素。结论：我们首次探讨了Raf-1作为下咽癌淋巴转移的独立预后危险因素。提示Raf-1可能作为下咽癌患者淋巴转移预防和诊断、改善患者预后的重要潜在生物标志物。
OBJECTIVE: We performed differential gene screening for lymphatic metastasis of hypopharyngeal carcinoma by gene sequencing. We also aimed to investigate the expression and clinicopathological significance of the screened gene in hypopharyngeal carcinoma lymphatic metastasis.METHODS: The clinicopathological characteristics of 98 patients with hypopharyngeal carcinoma were collected to make survival analysis by Kaplan-Meier & log-rank test. Six cases of tumor tissues from patients with or without lymphatic metastasis were used for gene sequencing of differentially expressed genes. The most frequently differently expressed genes were validated by RT-PCR and Western blot in another 20 patients diagnosed for hypopharyngeal carcinoma. A total of 70 cases of hypopharyngeal carcinoma tumor tissues and normal tissues were investigated to examine the immunohistochemical expression and to explore the prognostic value by Kaplan-Meier & log-rank test and Cox's test.RESULTS: Lymphatic metastasis has been proved to cause a reduction in postoperative survival of patients with hypopharyngeal carcinoma. The results of gene sequencing analysis showed that Raf-1 was a differentially expressed gene in lymphatic metastasis of hypopharyngeal carcinoma. Moreover, the expression of Raf-1 was significantly up-regulated in tumor tissues of lymphatic metastasis patients compared to non-lymphatic metastasis tumor tissues and normal tissues. Meanwhile, Raf-1 had been verified to be an independent risk factor affecting the prognosis of hypopharyngeal carcinoma.CONCLUSIONS: For the first time, we investigated Raf-1 as an independent prognostic risk factor of lymphatic metastasis in hypopharyngeal carcinoma. It suggests that Raf-1 may serve as an important potential biomarker in preventing and diagnosing lymphatic metastasis in patients with hypopharyngeal carcinoma and improving the prognosis of patients.