Protein folding liquid chromatography and its recent developments.

Protein folding liquid chromatography and its recent developments.
复制标题

DOI:
10.1016/j.jchromb.2006.10.068
复制
发表时间:
2007-04-15
期刊:
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
影响因子:
--
通讯作者:
Wang C
Wang C
中科院分区:
其他
文献类型:
--
作者:
Geng X;Wang C

文献摘要

参考文献

被引文献

相似文献

蛋白质组学的最终目标是识别生物活性蛋白质并使用细菌宿主等生物技术工具生产它们。然而,大肠杆菌产生的蛋白质必须重新折叠至其天然状态。蛋白质折叠液相色谱(PFLC)是近年来发展起来的新方法,广泛应用于分子生物学和生物技术领域。本文介绍了PFLC这一新方法,并对其近年来的发展进行了综述。此外,本文还包括 PFLC 的定义、优点、原理、实验室和大规模应用、设备和影响因素。此外,还探讨了该方法在未来的作用。
The ultimate goal of proteomics is to identify biologically active proteins and to produce them using biotechnology tools such as bacterial hosts. However, proteins produced by Escherichia coli must be refolded to their native state. Protein folding liquid chromatography (PFLC) is a new method developed in recent years, and it is widely used in molecular biology and biotechnology. In this paper, the new method, PFLC is introduced and its recent development is reviewed. In addition the paper includes definitions, advantages, principles, applications for both laboratory and large scales, apparatus, and effecting factors of PFLC. In addition, the role of this method in the future is examined.
DOI: 10.1007/s00449-001-0271-8
发表时间: 2001-11-01
影响因子: 3.8
作者:
Batas, B;Chaudhuri, JB
通讯作者: Chaudhuri, JB
DOI: 10.1023/a:1016305603569
发表时间: 2001-01-01
期刊: BIOSEPARATION
影响因子: --
作者:
Cho, TH;Ahn, SJ;Lee, EK
通讯作者: Lee, EK
DOI: 10.1016/s0378-4347(99)00360-6
发表时间: 2000-01-14
影响因子: 3
作者:
Fahey, EM;Chaudhuri, JB;Binding, P
通讯作者: Binding, P
DOI: 10.1016/0021-9673(94)80096-0
发表时间: 1994-05-06
影响因子: 4.1
作者:
FORCINITI, D
通讯作者: FORCINITI, D
DOI: 10.1038/6192
发表时间: 1999-02-01
影响因子: 46.9
作者:
Altamirano, MM;García, C;Fersht, AR
通讯作者: Fersht, AR