Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization
Characterization of Magnetic Nanoparticles Coated with Chitosan: A Potential Approach for Enzyme Immobilization
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DOI：
10.1155/2018/9468574
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发表时间：
2018-01-01
影响因子：
--
通讯作者：
Amaro-Reyes, Aldo
中科院分区：
文献类型：
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作者：
Diaz-Hernandez, Azariel;Gracida, Jorge;Amaro-Reyes, Aldo
磁性纳米粒子与蛋白质的交联在制备生物技术应用的新材料中起着重要作用。其目的是最大化的磁性质量吸引和蛋白质负载的磁性氧化铁纳米粒子包覆壳聚糖，合成在一个单一的步骤，通过碱沉淀。壳聚糖包覆的磁铁矿颗粒（Fe3O4@Chitosan）与木聚糖酶和纤维素酶（Fe3O4@Chitosan@Proteins）交联，显示出93%的磁铁矿磁饱和度。复合材料的X-射线衍射图对应于磁铁矿。热重分析和差示扫描量热分析表明，162毫克的壳聚糖是涂层的复合材料和12毫克的蛋白质交联到每克的磁性支持物的一个颗粒。通过傅里叶变换红外光谱、X射线能量和X射线光电子能谱分散分析证实了酶与Fe3O4@Chitosan之间的交联。根据动态光散射、透射和电子显微镜，Fe3O4@Chitosan和Fe3O4@Chitosan@Proteins的平均粒度分布分别为230 nm和430 nm，显示出单个球形颗粒的聚集体，平均直径分别为8.5 nm和10.8 nm。制备方法在决定超顺磁性纳米颗粒的尺寸和形状、尺寸分布、表面化学以及因此的应用中起着关键作用。
Cross-linking of magnetic nanoparticles with proteins plays a significant role in the preparation of new materials for biotechnological applications. The aim was the maximization of the magnetic mass attracted and protein loading of magnetic iron oxide nanoparticles coated with chitosan, synthesized in a single step by alkaline precipitation. Chitosan-coated magnetite particles (Fe3O4@Chitosan) were cross-linked to a xylanase and a cellulase (Fe3O4@Chitosan@Proteins), showing a 93% of the magnetic saturation of the magnetite. X-ray diffraction pattern in composites corresponds to magnetite. Thermogravimetry and differential scanning calorimetry showed that 162 mg of chitosan was coating one grain of composite and 12 mg of protein was cross-linked to each gram of magnetic support. Cross-linking between enzymes and Fe3O4@Chitosan was confirmed by infrared spectroscopy with Fourier transform, X-ray energy, and X-ray photoelectron spectroscopy dispersion analysis. From dynamic light scattering, transmission and electron microscopy the average particle size distribution was 230 nm and 430 nm for Fe3O4@Chitosan and Fe3O4@Chitosan@Proteins, showing agglomerates of individual spherical particles, with an average diameter of 8.5 nm and 10.8 nm, respectively. The preparation method plays a key role in determining the particle size and shape, size distribution, surface chemistry, and, therefore, the applications of the superparamagnetic nanoparticles.