Modulation of rat testicular LH/hCG receptors by membrane lipid fluidity
Modulation of rat testicular LH/hCG receptors by membrane lipid fluidity
复制标题
膜脂流动性对大鼠睾丸 LH/hCG 受体的调节
DOI：
10.1016/0303-7207(86)90107-3
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发表时间：
1986
影响因子：
4.1
通讯作者：
E. Šeböková
中科院分区：
文献类型：
--
作者：
J. Kolena;P. Blažíček;S. Horkovics;K. Ondrias;E. Šeböková
[125 I]hCG与大鼠睾丸膜的特异性结合被研究为脂质改变膜流动性的函数。用二苯基己三烯荧光去极化法或I（1，14），I（12，3）和CAT 16掺入膜的ESR谱测定膜流动性。胆固醇半琥珀酸酯孵育膜增加膜脂的刚性和[125 I]hCG的特异性结合。然而，饱和脂肪酸的类似硬化作用并不伴随着LH/hCG受体的可及性增加。用不饱和脂肪酸处理睾丸膜可增强膜的流动性，但促性腺激素的特异性结合消失。尽管用胆固醇半琥珀酸酯处理的细胞膜中LH/hCG受体增加，但Leydig细胞对cAMP响应LH刺激的敏感性降低。结果表明，新暴露的LH/hCG受体不与腺苷酸环化酶系统偶联。
The specific binding of [125I]hCG to rat testicular membrane preparations was investigated as a function of membrane fluidity changed by lipids. Membrane fluidity was measured either by fluorescence depolarization of diphenylhexatriene or ESR spectra of I(1,14), I(12,3) and CAT 16 incorporated into the membrane. Incubation of membrane with cholesteryl-hemisucinnate increased both the rigidity of membrane lipids and the specific binding of [125I]hCG. A similar rigidifying action of saturated fatty acids was, however, not accompanied by increased accessibility of LH/hCG receptors. Treatment of testicular membranes with unsaturated fatty acids enhanced membrane fluidity but specific binding of the gonadotropin disappeared. In spite of the increase of LH/hCG receptors in cell membranes treated with cholesteryl-hemisuccinate, Leydig cells showed decreased sensitivity to cAMP response to LH stimulation. The results indicate that newly exposed LH/hCG receptors are not coupled with the adenylate cyclase system.
乙醇对灵长类黄体膜的体外影响：额外促性腺激素受体位点的暴露。
影响因子：
4.8
作者：
Cameron,JL;Stouffer,RL
通讯作者：
Stouffer,RL