A continuous high-throughput bioparticle sorter based on 3D traveling-wave dielectrophoresis
A continuous high-throughput bioparticle sorter based on 3D traveling-wave dielectrophoresis
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DOI：
10.1039/b910587e
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Chang, Hsien-Chang
中科院分区：
文献类型：
--
作者：
Cheng, I-Fang;Froude, Victoria E.;Chang, Hsien-Chang
我们提出了一种高通量（最大流速类似于10 μ l/min或线速度类似于3 mm/s）连续生物颗粒分选机的基础上三维行波介电泳（twDEP）在500 kHz的最佳AC频率。高通量分选是通过垂直于连续通流的持续twDEP颗粒力来实现的，该连续通流通过单个3D电极阵列施加在整个芯片上。该设计允许基于交叉两侧的twDEP迁移率的差异将微米级颗粒连续分级到不同的下游子通道中。传统的DEP被整合到上游，以将颗粒集中到单个悬浮队列中，从而允许通过迁移率差异进行twDEP分选，并使沉降和场诱导的裂解最小化。3D电极阵列设计最小化了nDEP（具有朝向具有弱场的区域的粒子力的负DEP）对twDEP的抵消效应，使得两种力随着电压单调增加以进一步增加通量。证明了从碎片填充的异质样品中有效地聚焦和分离红细胞，以及将多分散的脂质体悬浮液基于尺寸分离成2.3至4.6 μ m和1.5至2.7 μ m的两个不同的条带，以6 μ l/min的手持芯片中记录的最高通量。
We present a high throughput (maximum flow rate similar to 10 mu l/min or linear velocity similar to 3 mm/s) continuous bio-particle sorter based on 3D traveling-wave dielectrophoresis (twDEP) at an optimum AC frequency of 500 kHz. The high throughput sorting is achieved with a sustained twDEP particle force normal to the continuous through-flow, which is applied over the entire chip by a single 3D electrode array. The design allows continuous fractionation of micron-sized particles into different downstream sub-channels based on differences in their twDEP mobility on both sides of the cross-over. Conventional DEP is integrated upstream to focus the particles into a single levitated queue to allow twDEP sorting by mobility difference and to minimize sedimentation and field-induced lysis. The 3D electrode array design minimizes the offsetting effect of nDEP (negative DEP with particle force towards regions with weak fields) on twDEP such that both forces increase monotonically with voltage to further increase the throughput. Effective focusing and separation of red blood cells from debris-filled heterogeneous samples are demonstrated, as well as size-based separation of poly-dispersed liposome suspensions into two distinct bands at 2.3 to 4.6 mu m and 1.5 to 2.7 mu m, at the highest throughput recorded in hand-held chips of 6 mu l/min.