Identification of the second major allergen of Japanese cedar pollen
Identification of the second major allergen of Japanese cedar pollen
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日本柳杉花粉第二大过敏原的鉴定
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
T. Matuhasi
中科院分区：
文献类型：
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作者：
Masahiro Sakaguchi;Sakae Inouye;M. Taniai;S. Ando;M. Usui;T. Matuhasi
我们从日本雪松花粉中分离并表征了第二种主要过敏原 (Cry j II)。我们发现大多数患有这种花粉病的患者除了针对 Cry j I 的 IgE 抗体外，还存在针对该蛋白的 IgE 抗体；然而，一些血清仅与 Cry j I 或 Cry j IL IgE-ELISA 反应抑制研究表明，Cry j I 和 Cry j II 没有交叉变应原性。 Cry j II 不与抗 Cry j I 单克隆抗体发生反应。在非还原条件下进行 SDS-PAGE。与Cry j I的45-50 kDa条带相比，Cry j II在37 kDa位置显示一条条带。Cry j II的N端氨基酸序列与Cry j I的N端氨基酸序列完全不同。
We isolated and characterized the second major allergen (Cry j II) from Japanese cedar pollen. We found that most patients with this pollinosis had IgE antibody to this protein in addition to IgE antibody to Cry j I; however, some sera reacted only with Cry j I or Cry j IL IgE‐ELISA inhibition studies revealed that Cry j I and Cry j II had no cross‐allergenicity. Cry j II did not react with anti‐Cry j I monoclonal antibodies. In SDS‐PAGE under a non‐reducing condition. Cry j II showed a band at the 37 kDa position, compared with the 45–50 kDa bands of Cry j I. N‐terminal amino acid sequence of Cry j II was completely different from that oi Cry j I.