MUTAGENIC RESPONSES OF NICKEL OXIDES AND NICKEL SULFIDES IN CHINESE-HAMSTER V79 CELL-LINES AT THE XANTHINE GUANINE PHOSPHORIBOSYL TRANSFERASE LOCUS
MUTAGENIC RESPONSES OF NICKEL OXIDES AND NICKEL SULFIDES IN CHINESE-HAMSTER V79 CELL-LINES AT THE XANTHINE GUANINE PHOSPHORIBOSYL TRANSFERASE LOCUS
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DOI：
10.1016/0165-1218(93)90141-y
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发表时间：
1993-06-01
期刊：
影响因子：
--
通讯作者：
COSTA, M
中科院分区：
文献类型：
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作者：
KARGACIN, B;KLEIN, CB;COSTA, M
在三种中国仓鼠细胞系中研究了几种不溶性镍化合物（结晶硫化镍 NiS、亚硫化镍 Ni3S2、氧化镍（黑色和绿色）和可溶性 NiCl2）的诱变作用 - 明确的 V79 细胞系的 hprt 基因以及两种转基因衍生细胞系 G12 和 G10 的 gpt 基因。转基因细胞系G12对不溶性Ni化合物的反应非常强烈，使得gpt诱变比自发诱变高至少20倍，并且在一些实验中甚至更高。相比之下，G10 细胞的反应要低得多——突变频率仅比对照组增加 2-3 倍。在 V79 细胞中，NiS 和 NiO（黑色）在 hprt 时不会诱导诱变反应。可溶性 NiCl2 在 V79 细胞中也没有表现出诱变活性，并且在转基因 G12 细胞中诱导的活性显着低于不溶性化合物。在暴露镍之前用维生素 E 对 G12 细胞进行 24 小时预处理后，观察到几种不溶性 Ni 化合物的细胞存活率增加，而维生素 E 对 NiCl2 细胞毒性没有影响。通过维生素 E 预处理，G12 细胞中某些不溶性 Ni 化合物的致突变反应也显着降低，而 NiCl2 则没有观察到这种效应。
Mutagenesis of several insoluble nickel compounds - crystalline nickel sulfide NiS, nickel subsulfide Ni3S2, nickel oxides (black and green) and soluble NiCl2 was studied in three Chinese hamster cell lines - at the hprt gene of the well-defined V79 cell line, and at gpt in two transgenic derivative cell lines G12 and G10. The transgenic cell line G12 responded very strongly to the insoluble Ni compounds, such that the gpt mutagenesis was at least 20 times higher than the spontaneous mutagenesis and in some experiments was even higher. In contrast the response of the G10 cells was much lower - the mutant frequencies only increased 2-3 times over the controls. In V79 cells, NiS and NiO (black) did not induce a mutagenic response at hprt. Soluble NiCl2 also exhibited no mutagenic activity in V79 cells and induced considerably lower activity than the insoluble compounds in the transgenic G12 cells.Following vitamin E pretreatment of G12 cells for 24 h prior to nickel exposure, increased cell survival was observed for several insoluble Ni compounds whereas vitamin E had no effect on NiCl2 cytotoxicity. With vitamin E pretreatment, significantly lower mutagenic responses in G12 cells were also noted for some insoluble Ni compounds, while no such effect was observed for NiCl2.