Molecular cloning, expression and characterization of the first three genes in the mevalonate-independent isoprenoid pathway in Streptomyces coelicolor
Molecular cloning, expression and characterization of the first three genes in the mevalonate-independent isoprenoid pathway in Streptomyces coelicolor
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DOI：
10.1016/s0968-0896(01)00050-5
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发表时间：
2001-06-01
影响因子：
3.5
通讯作者：
Kang, I
中科院分区：
文献类型：
--
作者：
Cane, DE;Chow, C;Kang, I
异戊烯基二磷酸和二甲基烯丙基二磷酸（类异戊二烯的通用前体）的甲羟戊酸盐非依赖性生物合成途径在真细菌（包括大肠杆菌）、藻类和高等植物的质体中起作用。检索桑格中心天蓝色链霉菌基因组数据库，发现开放阅读框与ca。与前三个E.大肠杆菌的酶，对应于脱氧木酮糖磷酸合成酶，脱氧木酮糖磷酸还原异构酶和2-C-甲基β 4-磷酸胞苷酰转移酶。色葡萄已克隆了腔棘鱼基因并在E.大肠杆菌，和重组蛋白的物理和动力学特征。结果表明，在S. coelicolor CH 999进一步支持该生物体中甲羟戊酸盐非依赖性途径的操作。(C)2001爱思唯尔科技有限公司版权所有。
The mevalonate-independent biosynthetic pathway to isopentenyl diphosphate and dimethylallyl diphosphate, the universal precursors to the isoprenoids, operates in eubacteria, including Escherichia coli, in algae, and in the plastids of higher plants. A search of the Sanger Centre Streptomyces coelicolor genome database revealed open reading frames with ca. 40-50% identity at the deduced amino acid level to the first three E. coli enzymes of this pathway, corresponding to deoxyxylulose phosphate synthase, deoxyxylulose phosphate reductoisomerase and 2-C-methyl erythritol 4-phosphate cytidylyltransferase. The S. coelicolor genes have been cloned and expressed in E. coli, and the recombinant proteins characterized physically and kinetically. The presence of the corresponding enzyme activities in extracts of S. coelicolor CH999 further supports the operation of the mevalonate-independent pathway in this organism. (C) 2001 Elsevier Science Ltd. All rights reserved.