NUCLEOLAR ORGANIZER OF PLETHODON-CINEREUS CINEREUS (GREEN) .1. LOCATION OF NUCLEOLAR ORGANIZER BY IN-SITU NUCLEIC-ACID HYBRIDIZATION
NUCLEOLAR ORGANIZER OF PLETHODON-CINEREUS CINEREUS (GREEN) .1. LOCATION OF NUCLEOLAR ORGANIZER BY IN-SITU NUCLEIC-ACID HYBRIDIZATION
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DOI：
10.1007/bf00399409
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发表时间：
1973-01-01
期刊：
影响因子：
1.6
通讯作者：
KEZER, J
中科院分区：
文献类型：
--
作者：
MACGREGOR, HC;KEZER, J
The 7th longest lampbrush bivalent in oocytes ofPlethodon cinereus cinereushas a region of attached oocyte nucleoli near to the centromere on the shorter arm of each half bivalent. When squash preparations ofP. c. cinereusspermatocytes are treated with NaOH to denature chromosomal DNA, and subsequently incubated in a solution of (3H) ribosomal RNA fromXenopuscell cultures, the ribosomal RNA binds specifically to a region near the centromeres on the shorter arms of the 7th longest bivalent, and to a region near the end of the shorter arm of the 14th bivalent. The amount of ribosomal RNA bound to the 7th bivalent at diplotene and 1st meiotic metaphase is regularly different on the 2 halves of the bivalent. Each half of the 14th bivalent is usually labelled more heavily than the less heavily labelled half of the 7th bivalent. These observations are discussed in relation to the involvement of nucleolar organizers in gene amplification and rectification.
