A simple method for screening photoelectric dyes towards their use for retinal prostheses.
A simple method for screening photoelectric dyes towards their use for retinal prostheses.
复制标题
一种筛选光电染料用于视网膜假体的简单方法。
DOI：
10.18926/amo/32824
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发表时间：
2003
影响因子：
0.5
通讯作者：
T. Matsuo
中科院分区：
文献类型：
--
作者：
T. Matsuo
光电染料吸收光并将光子能量转换成电势。为了测试这些染料是否可以用于视网膜假体，开发了一种简单的体外筛选系统。视网膜神经元培养的鸡胚胎的眼睛在10天的胚胎阶段，在这个时候还没有视网膜感光细胞的发展。用Fluo-4在培养的视网膜神经元中观察到细胞内钙升高，在将不同浓度的光电染料应用于培养基之前和之后。在这个体外系统中测试的7种光电染料中有5种诱导培养的鸡视网膜神经元细胞内钙升高。在所有5种染料的情况下，由5种光电染料产生的细胞内钙升高被细胞外钙耗尽阻断，并且除了一种染料之外，被电压门控钙通道阻断剂的存在阻断。光电染料在倒置显微镜下吸收光并刺激视网膜神经元。这种简单的体外系统允许筛选可用于视网膜假体的光电染料。
Photoelectric dyes absorb light and convert photon energy to electric potentials. To test whether these dyes could be used for retinal prostheses, a simple in vitro screening system was developed. Retinal neurons were cultured from the eyes of chick embryos at the 10-day embryonic stage, at which time no retinal photoreceptor cells have yet developed. Intracellular calcium elevation was observed with Fluo-4 in cultured retinal neurons before and after photoelectric dye was applied at varying concentrations to the culture medium. Five of 7 photoelectric dyes tested in this in vitro system induced intracellular calcium elevation in cultured chick retinal neurons. The intracellular calcium elevation generated by the 5 photoelectric dyes was blocked by extracellular calcium depletion in the case of all 5 dyes, and, except for one dye, by the presence of voltage-gated calcium channel blockers. The photoelectric dyes absorbed light under an inverted microscope and stimulated retinal neurons. This simple in vitro system allows the screening of photoelectric dyes which can be used for retinal prostheses.