Functional connectivity among ventrolateral medullary respiratory neurones and responses during fictive cough in the cat
Functional connectivity among ventrolateral medullary respiratory neurones and responses during fictive cough in the cat
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DOI：
10.1111/j.1469-7793.2000.00207.x
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发表时间：
2000-05-15
影响因子：
5.5
通讯作者：
Lindsey, BG
中科院分区：
文献类型：
--
作者：
Shannon, R;Baekey, DM;Lindsey, BG
1.本研究通过延髓腹外侧呼吸神经元相互作用的网络模型预测吸气和呼气泵肌肉中观察到的咳嗽运动模式。数据来自34只中丘去脑，瘫痪，人工通气猫。通过机械刺激胸内气管引起传出膈神经和腰神经活动的咳嗽样模式（虚构咳嗽）。腹侧呼吸组的神经元，包括Botzinger和pre-Botzinger复合体，同时监测微电极阵列。利用周期触发的直方图、自相关图、交叉相关图和膈神经和喉返神经活动的棘波触发平均值分析棘波序列，以获得虚构咳嗽期间功能连接和反应的证据。在1988对呼吸调制神经元中的151对中检测到显著的交叉相关图特征。吸气神经元对中有59个中央峰，5个中央谷，11个偏置峰和2个偏置谷。在呼气神经元中，有23个中央峰，8个偏移峰和4个偏移谷。吸气和呼气神经元之间的相关性包括20个中央峰，10个中央谷和9个偏移谷。膈神经运动神经元放电平均值有51个偏移峰和5个偏移谷.并发响应和多个短时间尺度的相关性支持并行和串行网络的相互作用，提出了在我们的模型中的呼吸泵肌肉的咳嗽运动模式的生成。推断的关联包括以下内容。(a)增强吸气（I-Aug）神经元和膈运动神经元的I-Aug神经元兴奋。(b)增强呼气（E-Aug）神经元和减少吸气（I-Dec）神经元的抑制。(c)E-Dec神经元对I-Aug、I-Dec和E-Aug神经元的抑制。(d)E-Aug神经元对I-Aug和I-Dec神经元以及膈运动神经元的抑制。这些数据也证实了以前的结果，并支持假设在目前的网络模型的产生eupnoeic模式。
1. This study tested predictions from a network model of ventrolateral medullary respiratory neurone interactions for the generation of the cough motor pattern observed in inspiratory and expiratory pump muscles.2. Data were from 34 mid-collicularly decerebrated, paralysed, artificially ventilated cats. Cough-like patterns (fictive cough) in efferent phrenic and lumbar nerve activities were elicited by mechanical stimulation of the intrathoracic trachea. Neurones in the ventral respiratory group, including the Botzinger and pre-Botzinger complexes, were monitored simultaneously with microelectrode arrays. Spike trains were analysed for evidence of functional connectivity and responses during fictive cough with cycle-triggered histograms, autocorrelograms, cross-correlograms, and spike-triggered averages of phrenic and recurrent laryngeal nerve activities.3. Significant cross-correlogram features were detected in 151 of 1988 pairs of respiratory modulated neurones. There were 59 central peaks, 5 central troughs, 11 offset peaks and 2 offset troughs among inspiratory neurone pairs. Among expiratory neurones there were 23 central peaks, 8 offset peaks and 4 offset troughs. Correlations between inspiratory and expiratory neurones included 20 central peaks, 10 central troughs and 9 offset troughs. Spike- triggered averages of phrenic motoneurone activity had 51 offset peaks and 5 offset troughs.4. The concurrent responses and multiple short time scale correlations support parallel and serial network interactions proposed in our model for the generation of the cough motor pattern in the respiratory pump muscles. Inferred associations included the following. (a) Excitation of augmenting inspiratory (I-Aug) neurones and phrenic motoneurones by I-Aug neurones. (b) Inhibition of augmenting expiratory (E-Aug) neurones blv decrementing inspiratory (I-Dec) neurones. (c) Inhibition of I-Aug, I-Dec and E-Aug neurones by E-Dec neurones. (d) Inhibition of I-Aug and I-Dec neurones and phrenic motoneurones by E-Aug neurones. The data also confirm previous results and support hypotheses in current network models for the generation of the eupnoeic pattern.