Polysaccharides from Polygonatum kingianum improve glucose and lipid metabolism in rats fed a high fat diet
Polysaccharides from Polygonatum kingianum improve glucose and lipid metabolism in rats fed a high fat diet
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黄精多糖改善高脂饮食大鼠的糖脂代谢
DOI：
10.1016/j.biopha.2020.109910
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发表时间：
2020-05-01
影响因子：
7.5
通讯作者：
Yu, Jie
中科院分区：
文献类型：
--
作者：
Gu, Wen;Wang, Yanfang;Yu, Jie
目的：黄精是治疗糖尿病和高脂血症的常用中药。方法：采用凝胶渗透色谱法（GPC）、液相色谱法（HPLC）和化学分析法分别测定黄精多糖的分子量、单糖组成、蛋白质、多糖和糖醛酸含量。分离总多糖（PS）和高分子量多糖（PSF）（> 100 KDa），并对HFD大鼠进行14周的治疗。每2周检测一次血中TC、TG含量，12周、14周检测HDL-C、LDL-C，评价多糖的调脂活性。采用16 S rDNA V4高变区测定法检测多糖对肠道菌群的调节作用。采用气相色谱法（GC）测定粪便中短链脂肪酸（SCFAs）的含量，探讨多糖对大鼠肠道SCFAs含量的影响。Western blot检测肠组织紧密连接蛋白ZO-1和occludin的表达，并检测LPS-TLR 4/NF kappa B信号通路中关键蛋白的表达。PS和PSF还调节了HFD大鼠的肠道微生物群组成、丰度和多样性，增加了短链脂肪酸（SCFA）产生菌的相对丰度并增加了SCFA的产生，降低了肠道通透性，减轻了胃肠道炎症，并改善了脂质代谢。黄精多糖通过增加肠道微生物群落的相对丰度来调节肠道微生物群落的丰度和多样性产生SCFA的细菌这促进了肠道通透性屏障的恢复，抑制LPS进入循环，减轻炎症，并防止葡萄糖和脂质代谢紊乱。
Objective: Polygonatum kingianum is a traditional Chinese medicine commonly used to treat diabetes and hyperlipidemia. Polysaccharides from Polygonatum kingianum could regulate glucose and lipid metabolism in high-fat diet (HFD) rats.Methods: The gel permeation chromatography (GPC), liquid chromatography (HPLC) and chemical analysis were used to determine the molecular weight, monosaccharide composition, protein, polysaccharide and uronic acid content of the polysaccharide from Polygonatum kingianum, respectively. Total (PS) and high molecular weight (PSF) polysaccharides (> 100 KDa) were isolated and treated HFD rats for a 14-week period. TC, TG contents in blood samples were measured every two weeks, and HDL-C, LDL-C was measured at 12 and 14 weeks evaluating of blood lipids regulation activity of polysaccharide. The stool samples were used for 16S rDNA V4 highly variable region measurement method to regulate the role of polysaccharide in the intestinal microflora. The content of short chain fatty acid (SCFAs) in faeces was determined by gas chromatography (GC) to investigate the effect of polysaccharides on the content of SCFAs in the intestinal tract of rats. The expression of tight junction protein ZO-1 and occludin of intestinal tissue, and detection of the key protein expression in the LPS-TLR4/NF kappa B signaling pathway were detected by Western blot technique.Results: We found that PS and PSF improved both diabetic symptoms and lipid metabolism. PS and PSF also modulated the gut microbiota composition, abundance and diversity of HFD rats, increased the relative abundance of short chain fatty acid (SCFA) producing bacteria and increased SCFA production, reduced intestinal permeability, relieved gastrointestinal inflammation, and improved lipid metabolism.Conclusion: Polygonatum kingianum polysaccharides regulated the abundance and diversity of the intestinal microbial community through increasing the relative abundance of SCFA-producing bacteria. This promotes recovery of the intestinal permeability barrier, inhibits LPS entry into the circulation, alleviates inflammation, and prevents glucose and lipid metabolic disorders.