Cardioprotective effects of Astragali radix against isoproterenol-induced myocardial injury in rats and its possible mechanism
Cardioprotective effects of Astragali radix against isoproterenol-induced myocardial injury in rats and its possible mechanism
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DOI：
10.1002/ptr.2332
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发表时间：
2008-03-01
影响因子：
7.2
通讯作者：
Cheng, Yan-Ping
中科院分区：
文献类型：
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作者：
Xu, Xiao-Le;Ji, Hui;Cheng, Yan-Ping
本研究旨在探讨中草药黄芪对在体心肌损伤的影响及其可能机制。大剂量异丙肾上腺素皮下注射致大鼠心肌损伤10d，观察黄芪的治疗作用。心脏血流动力学、心脏系数和血清标志物酶检测结果表明，黄芪对异丙肾上腺素所致心肌损伤有保护作用。黄芪还通过降低脂质过氧化产物丙二醛，提高抗氧化酶超氧化物歧化酶的活性，改善机体的抗氧化状态。黄芪可减轻异丙肾上腺素对大鼠肌浆网钙-三磷酸腺苷酶基因和蛋白表达的抑制以及Ser(16)磷酸化磷蛋白的表达。此外，与单纯异丙肾上腺素组相比，黄芪治疗组心肌cAMP含量更高。这些结果提示，黄芪的抗氧化作用和部分阻止心肌肌浆网钙调节蛋白蛋白和基因表达的变化可能是通过cAMP途径介导的，这可能有助于解释黄芪对在体心肌损伤的保护作用。版权所有(C)2007 John Wiley&Sons，Ltd.
The purpose of the present study was to investigate the effects of the Chinese medical herb Astragali Radix on myocardial injury in vivo and its possible mechanisms. Myocardial injury in rats was induced by the subcutaneous injection of a high dose of isoproterenol for 10 days, and the therapeutic effects of Astragali Radix were observed. Cardiac hemodynamics, heart coefficient and marker enzymes in serum showed that Astragali Radix prevented isoproterenol-induced myocardial damage. Astragali Radix also improved the antioxidant status by decreasing the lipid peroxidative product malondialdehyde and increasing the activity of the antioxidant enzyme superoxide dismutase. The observed depressions in sarcoplasmic reticulum Ca2+-ATPase mRNA and protein expression as well as Ser(16)-phosphorylated phospholamban protein expression in isoproterenol-treated rats were attenuated by Astragali Radix treatment. Moreover, treatment with Astragali Radix showed higher myocardial cAMP content compared with the isoproterenol-alone group. These results suggest that the antioxidant property and partial prevention of changes in protein and gene expression of cardiac sarcoplasmic reticulum Ca2+ regulatory proteins which may be mediated through the cAMP pathway could help to explain the beneficial effects of Astragali Radix on myocardial injury in vivo. Copyright (C) 2007 John Wiley & Sons, Ltd.