Nucleotide sequence of microvariant RNA: another small replicating molecule.
Nucleotide sequence of microvariant RNA: another small replicating molecule.
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微变体 RNA 的核苷酸序列：另一种小复制分子。
DOI：
10.1073/pnas.72.11.4252
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发表时间：
1975
影响因子：
11.1
通讯作者：
S. Speigelman
中科院分区：
文献类型：
--
作者：
D. Mills;F. Kramer;C. Dobkin;T. Nishihara;S. Speigelman
微变异RNA是一种自我复制的小分子（114个核苷酸长），已经从缺乏外源模板的qβ复制酶反应中分离出来。其完整的核苷酸序列已被确定。与dv -1 RNA比较，一个更大的内源性qβ复制酶产物（220个核苷酸长），之前已被表征，没有显着的序列相似性。由于qβ复制酶可以介导这两种不同RNA分子的合成，初级序列不能是酶识别和复制过程的唯一决定因素。这意味着关键是在二级或三级结构中找到的。确定序列的两种不同复制分子的可用性应该有助于确定这些关键的结构特征。
Microvariant RNA, a small self-replicating molecule (114 nucleotides long), has been isolated from Qbeta replicase reactions incubated in the absence of exogenous template. Its complete nucleotide sequence has been determined. Comparison with MDV-1 RNA, a somewhat larger endogenous Qbeta replicase product (220 nucleotides long) that had previously been characterized, revealed no significant sequence similarity. Since Qbeta replicase can mediate the synthesis of both of these disparate RNA molecules, primary sequence cannot be the sole determining factor in the processes of enzyme recognition and replication. This implies that the key is to be found in the secondary or tertiary structures. The availability of two different replicating molecules of defined sequence should aid in identifying these critical structural features.