Poly(ADP-ribose) polymerase 1 interacts with OAZ and regulates BMP-target genes
Poly(ADP-ribose) polymerase 1 interacts with OAZ and regulates BMP-target genes
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DOI：
10.1016/j.bbrc.2003.10.053
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发表时间：
2003-11-21
影响因子：
3.1
通讯作者：
Hata, A
中科院分区：
文献类型：
--
作者：
Ku, MC;Stewart, S;Hata, A
OAZ 是一种多锌指蛋白，可与包括 Olf-1/EBF、Smad1 和 Smad4 在内的多种不同核蛋白相互作用，并调节各种基因的转录。在这里，我们证明聚（ADP-核糖）聚合酶 1 (Parp1) 与 OAZ 形成复合物，并正向调节 BMP 靶基因、Xenopus Vent-2 和小鼠 Smad6 基因。 Parp1 的野生型和突变型（缺乏聚（ADP-核糖）聚合酶活性）均与 OAZ 发生组成性相互作用；然而，突变的 Parp1 并没有激活转录。这些结果表明聚（ADP-核糖）聚合酶活性对于 Vent-2 和 Smad6 的转录激活至关重要。这些结果表明 Parp1 在 BMP 依赖性基因调控中充当 OAZ 的转录共激活子。 (C) 2003 Elsevier Inc. 保留所有权利。
OAZ is a multi zinc finger protein which interacts with several distinct nuclear proteins including Olf-1/EBF, Smad1, and Smad4 and regulates transcription of various genes. Here we show that poly(ADP-ribose) polymerase 1 (Parp1) forms a complex with OAZ and positively regulates BMP-target genes, Xenopus Vent-2 and mouse Smad6 genes. Both wild type and the mutant forms of Parp1, which is deficient in poly(ADP-ribose) polymerase activity, constitutively interact with OAZ; however, the mutant Parp1 did not activate transcription. These results suggest that poly(ADP-ribose) polymerase activity is essential for the transcriptional activation of Vent-2 and Smad6. These results suggest that Parp1 serves as a transcriptional coactivator of OAZ in BMP-dependent gene regulation. (C) 2003 Elsevier Inc. All rights reserved.