Bee products prevent VEGF-induced angiogenesis in human umbilical vein endothelial cells.
Bee products prevent VEGF-induced angiogenesis in human umbilical vein endothelial cells.
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DOI：
10.1186/1472-6882-9-45
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发表时间：
2009-11-17
影响因子：
--
通讯作者：
Hara H
中科院分区：
文献类型：
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作者：
Izuta H;Shimazawa M;Tsuruma K;Araki Y;Mishima S;Hara H
血管内皮生长因子（VEGF）是诸如癌症和糖尿病视网膜病变等疾病中致病性血管生成的关键调节因子。来自意大利蜜蜂的蜂产品[皇家浆（RJ）、蜂花粉和中国红蜜蜂]几个世纪以来一直被用作传统的健康食品。本研究旨在探讨蜂产品对人脐静脉内皮细胞（HUVECs）血管生成的抑制作用。在体外试管形成试验中，将HUVEC和成纤维细胞与VEGF和各种浓度的蜂产品[RJ，蜂花粉的乙醇提取物，中国红枸杞的乙醇提取物及其成分咖啡酸苯乙酯（CAPE）]孵育14天。为了阐明体外血管生成的机制，VEGF与或不与不同浓度的RJ、蜂花粉、中国红色素和CAPE一起诱导HUVEC增殖和迁移。RJ、蜂花粉、中国红枸杞和CAPE对VEGF诱导的试管形成有显著的抑制作用，抑制作用大小顺序为：CAPE >中国红枸杞>>蜂花粉> RJ。RJ和中国红色素抑制VEGF诱导的HUVEC增殖和迁移。相反，蜂花粉和CAPE抑制增殖。蜂产品中，中国红蜂和CAPE尤其对VEGF诱导的血管生成具有较强的抑制作用。这些结果表明，中国红芪和CAPE可能是潜在的预防和治疗药物对血管生成相关的人类疾病。
Vascular endothelial growth factor (VEGF) is a key regulator of pathogenic angiogenesis in diseases such as cancer and diabetic retinopathy. Bee products [royal jelly (RJ), bee pollen, and Chinese red propolis] from the honeybee, Apis mellifera, have been used as traditional health foods for centuries. The aim of this study was to investigate the anti-angiogenic effects of bee products using human umbilical vein endothelial cells (HUVECs). In an in vitro tube formation assay, HUVECs and fibroblast cells were incubated for 14 days with VEGF and various concentrations of bee products [RJ, ethanol extract of bee pollen, ethanol extract of Chinese red propolis and its constituent, caffeic acid phenethyl ester (CAPE)]. To clarify the mechanism of in vitro angiogenesis, HUVEC proliferation and migration were induced by VEGF with or without various concentrations of RJ, bee pollen, Chinese red propolis, and CAPE. RJ, bee pollen, Chinese red propolis, and CAPE significantly suppressed VEGF-induced in vitro tube formation in the descending order: CAPE > Chinese red propolis >> bee pollen > RJ. RJ and Chinese red propolis suppressed both VEGF-induced HUVEC proliferation and migration. In contrast, bee pollen and CAPE suppressed only the proliferation. Among the bee products, Chinese red propolis and CAPE in particular showed strong suppressive effects against VEGF-induced angiogenesis. These findings indicate that Chinese red propolis and CAPE may have potential as preventive and therapeutic agents against angiogenesis-related human diseases.