KANADI regulates organ polarity in Arabidopsis
KANADI regulates organ polarity in Arabidopsis
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DOI：
10.1038/35079629
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发表时间：
2001-06-01
期刊：
影响因子：
64.8
通讯作者：
Poethig, RS
中科院分区：
文献类型：
--
作者：
Kerstetter, RA;Bollman, K;Poethig, RS
叶片和花器官沿其正面-背面（背-腹）轴极化。在拟南芥中，这种差异在前两个莲座叶中特别明显，它们的正面有毛状体（叶毛），但背面没有（1-3）。KANADI （KAN）突变等位基因在前两片叶子上产生背面毛状体的突变体中被鉴定出来。Kan突变最初被鉴定为螃蟹爪（crc）突变花表型的增强子，crc是一种指定心皮背面身份的基因（4,5）。在这里，我们发现KAN是叶片和心皮的背向身份所必需的，并且编码了GARP家族中一个核定位蛋白。KAN信使RNA的表达模式及在花菜花叶病毒（CAMV） 35S启动子调控下KAN异位表达的作用表明，KAN也可能在发育中的胚胎中指定外周身份。
Leaves and floral organs are polarized along their adaxial-abaxial (dorsal-ventral) axis. In Arabidopsis, this difference is particularly obvious in the first two rosette leaves, which possess trichomes (leaf hairs) on their adaxial surface but not their abaxial surface(1-3). Mutant alleles of KANADI (KAN) were identified in a screen for mutants that produce abaxial trichomes on these first two leaves. kan mutations were originally identified as enhancers of the mutant floral phenotype of crabs claw (crc), a gene that specifies abaxial identity in carpels(4,5). Here we show that KAN is required for abaxial identity in both leaves and carpels, and encodes a nuclear-localized protein in the GARP family of putative transcription factors. The expression pattern of KAN messenger RNA and the effect of ectopically expressing KAN under the regulation of the cauliflower mosaic virus (CAMV) 35S promoter indicate that KAN may also specify peripheral identity in the developing embryo.