Expression of CD47 (integrin-associated protein) decreases on red-blood cells during storage
Expression of CD47 (integrin-associated protein) decreases on red-blood cells during storage
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DOI：
10.1016/s1473-0502(02)00070-8
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发表时间：
2002-12-01
影响因子：
1.9
通讯作者：
Sparrow, RL
中科院分区：
文献类型：
--
作者：
Anniss, AM;Sparrow, RL
鉴于最近的研究表明CD47抗原是一种重要的自我识别标志，参与保护循环中的红细胞免受巨噬细胞的吞噬，本研究的目的是探讨红细胞在储存过程中CD47的表达是否发生了变化。分别于捐献后第1、14、28、42天采集标本，制备红细胞浓缩物(RCC)，4℃保存。用抗CD47抗体标记红细胞，用流式细胞仪进行分析。在保存14天的未照射和照射的红细胞中，观察到CD47抗原的表达有轻微但显著的进行性下降。储存14天后，RCCS上清液中CD47的含量也有所增加。目前认为，CD47在储存过程中的丢失，加上其他生物物理变化，可能是许多输血红细胞从受血者循环中清除的靶点。(C)2002爱思唯尔科学有限公司。保留所有权利。
In light of recent studies that demonstrate that CD47 antigen is an important "self-recognition" marker involved in protecting circulating red blood cells (RBCs) from phagocytosis by macrophages, the aim of the present study was to investigate whether CD47 expression on RBCs is altered during storage. Red cell concentrates (RCCs) were prepared and stored at 4,C and samples were collected on days 1, 14, 28 and 42 post donation. RBCs were labelled with anti-CD47 antibody and analysed by flow cytometry. A small but significant and progressive decrease in CD47 antigen expression was observed in non-irradiated and irradiated RBCs stored for 14 days onwards. CD47 was also detected in increasing quantities in supernatant from RCCs after 14 days of storage. It is proposed that loss of CD47 during storage, in addition to other biophysical changes, may target many transfused RBCs for clearance from the transfusion recipient's circulation. (C) 2002 Elsevier Science Ltd. All rights reserved.