The PAS/LOV protein VIVID supports a rapidly dampened daytime oscillator that facilitates entrainment of the Neurospora circadian clock
The PAS/LOV protein VIVID supports a rapidly dampened daytime oscillator that facilitates entrainment of the Neurospora circadian clock
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DOI：
10.1101/gad.349305
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发表时间：
2005-11-01
影响因子：
10.5
通讯作者：
Heintzen, C
中科院分区：
文献类型：
--
作者：
Elvin, M;Loros, JJ;Heintzen, C
粗糙脉孢菌的光生物钟控制其发育和生理。现有的简单模型重置的基础上光脉冲（所谓的非参数夹带）预测，恒定的光应该迅速发送时钟到一个pharmic状态，然而，这样的时钟将是一个有机体在野外改变光周期的用处不大，我们确认，真正的，虽然阻尼，节奏可以观察到在延长光。这种节律性需要PAS/LOV蛋白VIVID（VVD），该蛋白在光照下起作用，以促进振荡器的表达，该振荡器与在黑暗中运行的经典频率/白领复合体（FRQ/WCC）振荡器相关，但可与之区分。VVD阻止光重置时钟在黎明，但通过影响frq RNA周转，促进重置在黄昏，从而允许时钟运行通过黎明过渡，并采取其相位线索从黄昏。与此相一致，VVD的损失产生一个时钟，其性能遵循早期模型的简单预测，VVD的过度表达恢复了光的节律性和光周期的相位对Juration的敏感性。
A light-entrainable circadian clock controls development and physiology in Neurospora crassa. Existing simple models for resetting based on light pulses (so-called nonparametric entrainment) predict that constant light should quickly send the clock to an arrhythmic state; however, such a clock would be of little use to an organism in changing photoperiods in the wild, and we confirm that true, albeit dampened, rhythmicity can be observed in extended light. This rhythmicity requires the PAS/LOV protein VIVID (VVD) that acts, in the light, to facilitate expression of an oscillator that is related to, but distinguishable from, the classic FREQUENCY/WHITE-COLLAR complex (FRQ/WCC)-based oscillator that runs in darkness. VVD prevents light resetting of the clock at dawn but, by influencing frq RNA turnover, promotes resetting at dusk, thereby allowing the clock to run through the dawn transition and take its phase cues from dusk. Consistent with this, loss of VVD yields a clock whose performance follows the simple predictions of earlier models, and overexpression of VVD restores rhythmicity in the light and sensitivity of phase to the Juration of the photoperiod.