A targeted gene silencing technique shows that Drosophila myosin VI is required for egg chamber and imaginal disc morphogenesis.
A targeted gene silencing technique shows that Drosophila myosin VI is required for egg chamber and imaginal disc morphogenesis.
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靶向基因沉默技术表明果蝇肌球蛋白 VI 是卵室和成虫盘形态发生所必需的。
DOI：
10.1242/jcs.112.21.3677
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发表时间：
1999
影响因子：
4
通讯作者：
["Wu
中科院分区：
文献类型：
--
作者：
["Wu
我们报告，果蝇非常规肌球蛋白VI，编码的肌球蛋白重链在95 F（Mhc 95 F），是所需的成虫盘和卵室形态发生。在卵子发生过程中，Mhc 95 F在迁移卵泡细胞中表达，包括边缘细胞，其在保育细胞之间迁移以位于卵母细胞的前部;在卵母细胞上方迁移的柱状细胞;在卵母细胞和保育细胞之间迁移的向心细胞;以及背前卵泡细胞，其迁移以分泌绒毛膜附件。利用Gal 4-UAS靶向表达系统和反义RNA技术，结合靶向基因沉默技术，研究了其在发育过程中的功能。抗体染色结果显示，表达反义Mhc 95 F RNA后，卵泡细胞中肌球蛋白95 F的表达明显降低。在不同发育阶段干扰果蝇肌球蛋白VI的表达经常导致死亡。在变态过程中，它会导致成年苍蝇畸形的腿和翅膀，表明肌球蛋白VI是至关重要的成虫盘形态发生。在卵子发生过程中，观察到异常的卵泡细胞形状和异常的卵泡细胞迁移时，反义Mhc 95 F在卵泡细胞中表达的阶段9至10，这表明果蝇肌球蛋白VI是所需的卵泡细胞上皮形态发生。
We report that Drosophila unconventional myosin VI, encoded by Myosin heavy chain at 95F (Mhc95F), is required for both imaginal disc and egg chamber morphogenesis. During oogenesis, Mhc95F is expressed in migrating follicle cells, including the border cells, which migrate between the nurse cells to lie at the anterior of the oocyte; the columnar cells that migrate over the oocyte; the centripetal cells that migrate between the oocyte and nurse cells; and the dorsal-anterior follicle cells, which migrate to secrete the chorionic appendages. Its function during development has been studied using a targeted gene silencing technique, combining the Gal4-UAS targeted expression system and the antisense RNA technique. Antibody staining shows that the expression of myosin 95F is greatly decreased in follicle cells when antisense Mhc95F RNA is expressed. Interfering with expression of Drosophila myosin VI at various developmental stages frequently results in lethality. During metamorphosis it results in adult flies with malformed legs and wings, indicating that myosin VI is essential for imaginal disc morphogenesis. During oogenesis, abnormal follicle cell shapes and aberrant follicle cell migrations are observed when antisense Mhc95F is expressed in follicle cells during stages 9 to 10, suggesting that the Drosophila myosin VI is required for follicle cell epithelial morphogenesis.
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影响因子：
4.6
作者：
XU Tian;Laurent A. Caron;R. Fehon;S. Artavanis-Tsakonas
通讯作者：
XU Tian;Laurent A. Caron;R. Fehon;S. Artavanis-Tsakonas
脑神经源性位点与果蝇 EGF 受体合作建立卵泡并确定其背腹极性。
DOI：
10.1242/dev.116.1.177
发表时间：
1992
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Goode,S;Wright,D;Mahowald,AP
通讯作者：
Mahowald,AP
影响因子：
4.6
作者：
S. Goode;M. Melnick;T. Chou;N. Perrimon
通讯作者：
S. Goode;M. Melnick;T. Chou;N. Perrimon
Ras 在发育调控细胞迁移中发挥两种不同的作用。
DOI：
10.1242/dev.122.2.409
发表时间：
1996
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Lee,T;Feig,L;Montell,DJ
通讯作者：
Montell,DJ