Comparative in vitro cytotoxicity assessment of some manufactured nanoparticulate materials characterized by transmission electron microscopy
Comparative in vitro cytotoxicity assessment of some manufactured nanoparticulate materials characterized by transmission electron microscopy
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DOI：
10.1007/s11051-005-3473-1
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发表时间：
2005-06-01
影响因子：
2.5
通讯作者：
Murr, LE
中科院分区：
文献类型：
--
作者：
Soto, KF;Carrasco, A;Murr, LE
一系列人造纳米颗粒材料，包括 Ag、TiO2、Fe2O3、Al2O3、ZrO2、Si3N4 和一系列碳质纳米颗粒材料：单壁和多壁碳纳米管聚集体和黑碳聚集纳米颗粒，以及商业（矿物级）温石棉纳米管聚集体，已通过透射电子显微镜进行了严格表征。这些纳米颗粒材料的初级粒径范围约为 3 至 150 nm（长度超过 15 μm 的纳米管材料除外）。聚集体尺寸范围为 25 nm 至 20 μm。利用鼠（肺）巨噬细胞系对这些纳米材料进行了比较细胞毒理学评估。以温石棉为阳性对照，并指定其相对细胞毒性指数为1（1.0），在5μg/ml浓度下观察到的相对细胞毒性指数如下：Ag和TiO2分别为1.6和0.4； Fe2O3、Al2O3和ZrO2为0.7-0.9，Si3N4为0.4，黑碳为0.8，碳纳米管聚集体样品为0.9至1.1。多壁碳纳米管聚集体与环境中的人造多壁碳纳米管非常相似，观察到的细胞毒性反应几乎与温石棉相同，引起了人们对潜在健康影响的担忧，特别是长期接触。
A range of manufactured nanoparticulate materials, including Ag, TiO2, Fe2O3, Al2O3, ZrO2, Si3N4, and a range of carbonaceous nanoparticulate materials: single-wall and multi-wall carbon nanotube aggregates and aggregated nanoparticles of black carbon, as well as commercial (mineral grade) chrysotile asbestos nanotube aggregates, have been rigorously characterized by transmission electron microscopy. These nanoparticulate materials ranged in primary particle sizes from roughly 3 to 150 nm (except for the nanotube materials with lengths in excess of 15 mu m). Aggregate sizes ranged from 25 nm to 20 mu m. Comparative cytotoxicological assessment of these nanomaterials was performed utilizing a murine (lung) macrophage cell line. Considering the chrysotile asbestos to be a positive control, and assigning it a relative cytotoxicity index of unity (1.0), relative cytotoxicity indexes were observed as follows at concentrations of 5 mu g/ml: 1.6 and similar to 0.4 for Ag and TiO2, respectively; 0.7-0.9 for the Fe2O3, Al2O3 and ZrO2, 0.4 for the Si3N4, 0.8 for the black carbon, and 0.9 to 1.1 for the carbon nanotube aggregate samples. Observations of a cytotoxic response, nearly identical to that for chrysotile asbestos, for multi-wall carbon nanotube aggregates which very closely resemble anthropogenic multi-wall carbon nanotubes in the environment, raise some concern for potential health effects, especially for long-term exposure.