TRANSFER-RNA GENES FROM DICTYOSTELIUM-DISCOIDEUM ARE FREQUENTLY ASSOCIATED WITH REPETITIVE ELEMENTS AND CONTAIN CONSENSUS BOXES IN THEIR 5'-FLANKING AND 3'-FLANKING REGIONS
TRANSFER-RNA GENES FROM DICTYOSTELIUM-DISCOIDEUM ARE FREQUENTLY ASSOCIATED WITH REPETITIVE ELEMENTS AND CONTAIN CONSENSUS BOXES IN THEIR 5'-FLANKING AND 3'-FLANKING REGIONS
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DOI：
10.1016/0022-2836(91)90495-r
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发表时间：
1991-12-05
影响因子：
5.6
通讯作者：
DINGERMANN, T
中科院分区：
文献类型：
--
作者：
HOFMANN, J;SCHUMANN, G;DINGERMANN, T
A total of 68 different tRNA genes from the cellular slime moldDictyostelium discoideumhave been isolated and characterized. Although these tRNA genes show features common to typical nuclear tRNA genes from other organisms, several unique characteristics are apparent: (1) the 5′-proximal flanking region is very similar for most of the tRNA genes; (2) more than 80% of the tRNA genes contain an “ex-B motif” within their 3′-flanking region, which strongly resembles characteristics of the consensus sequence of a T-stem/T-loop region (B-box) of a tRNA gene; (3) probably more than 50% of the tRNA genes in certainD. discoideumstrains are associated with a retrotransposon, termed DRE (Dictyosteliumrepetitive element), or with a transposon, termed Tdd-3 (TransposonDictyostelium discoideum). DRE always occurs 50(±3) nucleotides upstream and Tdd-3 always occurs 100(± 20) nucleotides downstream from the tRNA gene.D. discoideumtRNA genes are organized in multicopy gene families consisting of 5 to 20 individual genes. Members of a particular gene family are identical within the mature tRNA coding region while flanking sequences are idiosyncratic.
