Synaptic efficacy enhanced by glial cells in vitro
Synaptic efficacy enhanced by glial cells in vitro
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DOI：
10.1126/science.277.5332.1684
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发表时间：
1997-09-12
期刊：
影响因子：
56.9
通讯作者：
Barres, BA
中科院分区：
文献类型：
--
作者：
Pfrieger, FW;Barres, BA
在发育中的神经系统中，神经胶质细胞引导轴突到达其靶区域，但尚不清楚它们是否帮助神经元建立功能性突触连接。在来自大鼠中枢神经系统的纯化神经元的培养物中研究了神经胶质细胞在突触形成和功能中的作用。在无神经胶质细胞的培养物中，视网膜神经节细胞形成具有正常超微结构的突触，但在诱发性突触传递中显示很少的自发性突触活动和高的失败率。在与神经胶质细胞的共培养中，自发突触后电流的频率和幅度分别增强了70倍和5倍，并且发生较少的传递失败。胶质细胞使动作电位非依赖性的关醛释放增加了12倍，而不影响神经元的存活。
In the developing nervous system, glial cells guide axons to their target areas, but it is unknown whether they help neurons to establish functional synaptic connections, The role of glial cells in synapse formation and function was studied in cultures of purified neurons from the rat central nervous system, In glia-free cultures, retinal ganglion cells formed synapses with normal ultrastructure but displayed little spontaneous synaptic activity and high failure rates in evoked synaptic transmission, In cocultures with neuroglia, the frequency and amplitude of spontaneous postsynaptic currents were potentiated by 70-fold and 5-fold, respectively, and fewer transmission failures occurred, Glial cells increased the action potential-independent guantal release by 12-fold without affecting neuronal survival, Thus, developing neurons in culture form inefficient synapses that require glial signals to become fully functional.