Apoptosis of mouse pancreatic acinar cells after duct ligation.
Apoptosis of mouse pancreatic acinar cells after duct ligation.
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导管结扎后小鼠胰腺腺泡细胞的凋亡。
DOI：
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发表时间：
1995
影响因子：
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通讯作者：
S. Watanabe
中科院分区：
文献类型：
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作者：
K. Abe;S. Watanabe
已经确定胰管阻塞后胰腺外分泌腺泡细胞消失。本研究旨在探讨六周龄 dd 小鼠脾叶胰管结扎后腺泡细胞消失与细胞凋亡之间的关系。在一些小鼠中，结扎线在两三天后被移除。除了对胰腺组织进行一般光学和电子显微镜检查外，还观察用末端脱氧核苷酸转移酶介导的 dUTP-生物素缺口末端标记 (TUNEL) 方法染色的石蜡切片以检测核 DNA 碎片。胰管结扎后三天，胰腺腺泡细胞因核DNA损伤而发生凋亡，并在七天后完全消失。由于腔内压力升高，腺泡细胞的酶原颗粒分泌中断，粗面内质网紊乱，导致细胞凋亡开始前颗粒形成停止。正如在去除连接后观察到的，细胞凋亡之前的这些细胞质变化是可逆的。腺泡细胞的凋亡通过细胞核的 TUNEL 标记、核染色质的浓缩和边缘化以及细胞体的圆形碎片（所有这些都是不可逆的变化）来鉴定。腺泡细胞的凋亡似乎刺激了导管细胞的增殖，导管细胞是腺泡细胞消失后胰腺外分泌的主要细胞成分。
It has been established that pancreatic exocrine acinar cells disappear after pancreatic duct obstruction. This study aimed to examine the relationship between the disappearance of the acinar cells and apoptosis after pancreatic duct ligation of the splenic lobe in dd-mice, six weeks of age. In some mice, the ligature was removed after two or three days. In addition to general light and electron microscopic examinations on the pancreatic tissues, paraffin sections stained with the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) method were observed to detect nuclear DNA fragmentation. Pancreatic acinar cells underwent apoptosis initiated with nuclear DNA damages three days after duct ligation and were completely deleted by seven days. Due to the elevation of the intraluminal pressure, the acinar cells showed interrupted secretion of their zymogen granules and disorganization of their rough endoplasmic reticulum, causing the cessation of granule formation before apoptosis started. These cytoplasmic changes prior to apoptosis are reversible, as observed after removal of the ligation. Apoptosis of the acinar cells was indentified by TUNEL-labeling of the nuclei, the condensation and margination of nuclear chromatin, and round fragmentation of cell bodies, all irreversible changes. Apoptosis of acinar cells seemed to stimulate the proliferation of duct cells, which comprised the main cell components in the exocrine pancreas after the disappearance of acinar cells.