Assembly and remodeling of the fibrillar fibronectin extracellular matrix during gastrulation and neurulation in Xenopus laevis
Assembly and remodeling of the fibrillar fibronectin extracellular matrix during gastrulation and neurulation in Xenopus laevis
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DOI：
10.1002/dvdy.20217
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发表时间：
2004-12-01
影响因子：
2.5
通讯作者：
DeSimone, DW
中科院分区：
文献类型：
--
作者：
Davidson, LA;Keller, R;DeSimone, DW
纤维连接蛋白是细胞外基质的主要成分，对细胞牵引和细胞运动过程至关重要。整体安装共聚焦成像的三维结构的细胞外基质被用来描述动态组装和重塑纤连蛋白原纤维在原肠胚和神经胚形成早期青蛙胚胎。如前所述，原纤维首先出现在预期的外胚层下。我们在这里描述的第一个证据，为管制组装的原纤维沿着体节中胚层/内胚层边界，以及在脊索/体节中胚层边界和清除原纤维从背侧和腹面的脊索，发生在几个小时的过程。随着原肠胚形成的进行，原纤维恢复到脊索的背表面，在那里脊索接触预期的底板。当神经褶形成时，原纤维再次被重塑，因为深层神经板细胞向内侧移动。神经管闭合的过程留下覆盖神经嵴的外胚层区域，该区域暂时没有原纤维。当神经管腔恢复时，原纤维围绕神经管的背侧表面组装。(C)2004 Wiley-Liss，Inc.
Fibronectin, a major component of the extracellular matrix is critical for processes of cell traction and cell motility. Whole-mount confocal imaging of the three-dimensional architecture of the extracellular matrix is used to describe dynamic assembly and remodeling of fibronectin fibrils during gastrulation and neurulation in the early frog embryo. As previously reported, fibrils first appear under the prospective ectoderm. We describe here the first evidence for regulated assembly of fibrils along the somitic mesoderm/endoderm boundary as well as at the notochord/somitic mesoderm boundary and clearing of fibrils from the dorsal and ventral surfaces of the notochord that occurs over the course of a few hours. As gastrulation proceeds, fibrils are restored to the dorsal surface of the notochord, where the notochord contacts the prospective floor plate. As the neural folds form, fibrils are again remodeled as deep neural plate cells move medially. The process of neural tube closure leaves a region of the ectoderm overlying the neural crest transiently bare of fibrils. Fibrils are assembled surrounding the dorsal surface of the neural tube as the neural tube lumen is restored. (C) 2004 Wiley-Liss, Inc.