The interaction of the SARS coronavirus non-structural protein 10 with the cellular oxido-reductase system causes an extensive cytopathic effect.
The interaction of the SARS coronavirus non-structural protein 10 with the cellular oxido-reductase system causes an extensive cytopathic effect.
复制标题
DOI：
10.1016/j.jcv.2004.12.019
复制
发表时间：
2005-10
期刊：
影响因子：
--
通讯作者：
Ma S
中科院分区：
文献类型：
--
作者：
Li Q;Wang L;Dong C;Che Y;Jiang L;Liu L;Zhao H;Liao Y;Sheng Y;Dong S;Ma S
探讨SARS-CoV感染的病理机制。合成了位于pp 1a/pp 1ab基因开放阅读框中的SARS冠状病毒非结构蛋白10（non-structural protein 10，NSP 10）基因，并利用酵母诱捕法从人胚肺cDNA文库中筛选编码与NSP 10蛋白相互作用的特异性细胞基因。结果表明，nsp 10蛋白除了与细胞RNA聚合酶复合物的两个亚基BTF 3和ATF 5相互作用外，还能与细胞色素氧化酶II和NADH 4L亚基相互作用。进一步的研究表明，nsp 10蛋白基因转染人胚肺成纤维细胞后，细胞内NADH-细胞色素活性发生改变，线粒体内膜发生去极化。冠状病毒229 E株在这些细胞中的致细胞病变效应似乎比对照细胞更广泛。
The pathological mechanism of SARS-CoV infection was investigated. The gene for the SARS-CoV non-structural protein 10, which is located in the open reading frame of pp1a/pp1ab gene, was synthesized and used to screen for the specific cellular gene coding for the protein interacting with this nsp10 protein in a human embryo lung cDNA library using a yeast trap method. The results indicated that apart from the two subunits of cellular RNA polymerase complex, BTF3 and ATF5, this nsp10 protein was also able to interact specifically with the NADH 4L subunit and cytochrome oxidase II. Further study revealed that the activity of the NADH-cytochrome was altered and the inner mitochondrial membrane was depolarized in the transfected human embryo lung fibroblast by the nsp10 protein gene. The cytopathic effect of the Coronavirus 229E strain appeared more extensive in these cells than in the control cells.
登录
查看更多内容
影响因子：
56.9
作者：
Anand, K;Ziebuhr, J;Hilgenfeld, R
通讯作者：
Hilgenfeld, R
影响因子：
1.6
作者：
SLATER, M
通讯作者：
SLATER, M
DOI：
10.1073/pnas.76.9.4350
发表时间：
1979-01-01
影响因子：
11.1
作者：
TOWBIN, H;STAEHELIN, T;GORDON, J
通讯作者：
GORDON, J
DOI：
10.1001/jama.289.21.joc30885
发表时间：
2003-06-04
影响因子：
120.7
作者：
Booth, CM;Matukas, LM;Detsky, AS
通讯作者：
Detsky, AS
DOI：
10.1016/s0140-6736(03)13412-5
发表时间：
2003-05-24
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Chu CM;Cheng VC;Chan KS;Hung IF;Poon LL;Law KI;Tang BS;Hon TY;Chan CS;Chan KH;Ng JS;Zheng BJ;Ng WL;Lai RW;Guan Y;Yuen KY;HKU/UCH SARS Study Group
通讯作者：
HKU/UCH SARS Study Group