MORPHOLOGICAL ORGANIZATION OF RAT HIPPOCAMPAL SLICE CULTURES
MORPHOLOGICAL ORGANIZATION OF RAT HIPPOCAMPAL SLICE CULTURES
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DOI：
10.1002/cne.903070109
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发表时间：
1991-05-01
影响因子：
2.5
通讯作者：
AERTSEN, AD
中科院分区：
文献类型：
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作者：
CAESER, M;AERTSEN, AD
使用各种组织学方法，我们研究了大鼠海马脑片培养的细胞和形态组织。海马体的许多典型特征在体外保留了很长一段时间。海马和齿状回的主要细胞类型（锥体细胞和颗粒细胞）在体外保存完好并成熟。非锥体细胞和γ-氨基丁酸（GABA）细胞也存在于切片培养，并表现出惊人的相似的树突状外观在光镜水平。此外，GABA免疫反应阳性的细胞体和突触前末梢可以确定在电子显微镜水平，他们表示典型的对称突触接触细胞体和树突。内在海马纤维路径的苔藓纤维，谢弗侧支和肺泡的过程中，一般保留在体外。可以识别出额外的异常纤维投射。最后，三种类型的非神经元细胞可以区分免疫细胞化学方法的基础上。
Using various histological methods, we investigated the cellular and morphological organization of rat hippocampal slice cultures. Many of the typical features of the hippocampus were retained in vitro over a long period of time. The principal cell types of the hippocampus and dentate gyrus, the pyramidal cells and granule cells, were well preserved and matured in vitro. Nonpyramidal cells and gamma-aminobutyric-acid (GABA) cells were also present in slice cultures and exhibited a strikingly similar dendritic appearance at the light microscopic level. Moreover, GABA-immunoreactive cell bodies and presynaptic terminals could be identified at the electron microscopic level; they expressed typical symmetric synaptic contacts with cell bodies and dendrites. The course of the intrinsic hippocampal fiber pathways-the mossy fibers, Schaffer collaterals, and alveus-was generally retained in vitro. Additional aberrant fiber projections could be identified. Finally, three types of nonneuronal cells could be distinguished on the basis of immunocytochemical methods.