Experimental challenge of Anisakis simplex sensu stricto and Anisakis pegreffii (Nematoda: Anisakidae) in rainbow trout and olive flounder
Experimental challenge of Anisakis simplex sensu stricto and Anisakis pegreffii (Nematoda: Anisakidae) in rainbow trout and olive flounder
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DOI：
10.1016/j.parint.2010.11.007
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发表时间：
2011-06-01
影响因子：
1.9
通讯作者：
Ogawa, Kazuo
中科院分区：
文献类型：
--
作者：
Quiazon, Karl Marx A.;Yoshinaga, Tomoyoshi;Ogawa, Kazuo
本文报道了广义异尖线虫（Anisakis simplex lato（s.l.）存在于许多海洋鱼类中。为确保食物安全，确定这些幼虫是否存在于商业鱼类的身体肌肉中非常重要。然而，关于异尖线虫及其两个兄弟种异尖线虫的组织特异性的信息很少。狭义单纯形以及在日本沃茨的海鱼中常见的异尖线虫。我们用这两个兄弟物种的L3幼虫经口挑战虹鳟鱼（Oncorhynchus mykiss（Walbaum））和橄榄比目鱼（Paralichthys olivaceus（Temminck和Schlegel））并监测感染5周。虹鳟A.单纯性s.s.，而不是A。pegreffii幼虫，迁移到身体肌肉。少量自由移动的A.在体腔内回收了佩雷菲幼虫。橄榄比目鱼A.单纯型在体腔和肌肉中均发现幼虫。A. pegreffii幼虫仅在体腔中发现，并且主要被包裹在肿块中。我们的结果表明，两个姊妹种之间的感染位点和宿主特异性存在差异。辛普莱普公司(C)2010爱思唯尔爱尔兰有限公司版权所有。
The third-stage larvae of Anisakis simplex sensu lato (s.l.) are found in many marine fishes. To ensure food safety, it is important to determine whether these larvae are present in the body muscle of commercial fish species. However, there is little information regarding the tissue specificity of Anisakis and two of its sibling species, A. simplex sensu stricto (s.s.) and Anisakis pegreffii, that are common in marine fish in Japanese waters. We orally challenged rainbow trout (Oncorhynchus mykiss (Walbaum)), and olive flounder (Paralichthys olivaceus (Temminck and Schlegel)) with L3 larvae of these two sibling species and monitored infection for 5 weeks. In rainbow trout, A. simplex s.s., but not A. pegreffii larvae, migrated into the body muscle. A small number of freely moving A. pegreffii larvae were recovered within the body cavity. In olive flounder, A. simplex s.s. larvae were found in both the body cavity and body muscle. A. pegreffii larvae were found only in the body cavity and primarily encapsulated in lumps. Our results indicate that there are differences in the sites of infection and host specificity between the two sibling species of A. simplex s.l. (C) 2010 Elsevier Ireland Ltd. All rights reserved.