Tryptic peptide analysis of avian oncovirus gag and pol gene products
Tryptic peptide analysis of avian oncovirus gag and pol gene products
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禽肿瘤病毒 gag 和 pol 基因产物的胰蛋白酶肽分析
DOI：
10.1128/jvi.32.1.102-113.1979
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发表时间：
1979
影响因子：
5.4
通讯作者：
H. Hanafusa
中科院分区：
文献类型：
--
作者：
C. W. Rettenmier;R. Karess;S. Anderson;H. Hanafusa
研究了禽癌病毒gag和pol基因产物的放射标记色氨酸。分析了劳斯相关病毒2型感染鸡胚细胞的结构蛋白以及Pr76gag和P180gag-pol蛋白。Pr76gag和P180gag-pol中均含有病毒粒子内部结构蛋白中含有蛋氨酸和半胱氨酸的色氨酸肽，说明在P180gag-pol的生成过程中，gag基因编码序列没有丢失。gag和pol基因结构信息未发现重叠。对Pr76gag加工过程中的中间体和与帕霉素的翻译抑制定位进行分析，得出劳斯相关病毒2 Pr76gag前体的结构蛋白顺序如下：NH2-p19-p12-p27-p15-COOH。通过与rouss相关病毒2 P180gag-pol的比较，鉴定了未感染gs+鸡细胞内源性gsmp120蛋白缺失的gag和pol序列。
Radiolabeled tryptic peptides of the gag and pol gene products of avian oncoviruses were examined. This analysis included Rous-associated virus 2 structural proteins and the Pr76gag and P180gag-pol proteins in Rous-associated virus 2-infected chicken embryo cells. The methionine- and cysteine-containing tryptic peptides of virion internal structural proteins were present in both Pr76gag and P180gag-pol, suggesting that there was no loss of gag gene-coding sequences during the generation of P180gag-pol. No overlap of gag and pol gene structural information was detected. Analysis of intermediates in the processing of Pr76gag and translation inhibition mapping with pactamycin yielded the following order of structural proteins within the Rous-associated virus 2 Pr76gag precursor: NH2-p19-p12-p27-p15-COOH. The gag and pol sequences missing in the endogenous gsmp120 protein of uninfected gs+ chicken cells were identified by comparison with those of Rous-associated virus 2 P180gag-pol.