The roles of IL-19 and IL-20 in the inflammation of degenerative lumbar spondylolisthesis.
The roles of IL-19 and IL-20 in the inflammation of degenerative lumbar spondylolisthesis.
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DOI：
10.1186/s12950-018-0195-6
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Lin RM
中科院分区：
文献类型：
--
作者：
Huang KY;Hsu YH;Chen WY;Tsai HL;Yan JJ;Wang JD;Liu WL;Lin RM
退行性腰椎滑脱（DLS）是椎管狭窄的主要原因，通常与下背痛有关。IL-20是一种与椎间盘突出、类风湿性关节炎（RA）、骨质疏松和骨折等多种慢性炎症性骨病相关的强效血管生成、趋化和促炎细胞因子。IL-19也在RA中作为促炎细胞因子。本研究的目的是研究IL-19和IL-20是否参与DLS，并比较DLS患者的椎间盘、小关节和黄韧带等三种不同组织，以验证哪种组织更容易受到炎症的影响。取13例DLS患者的椎间盘、小关节和黄韧带，采用免疫组织化学方法检测IL-19、IL-20、IL-20 R1、IL-20 R2、TNF-α、IL-1β和MCP-1的表达。分离椎间盘细胞，并在CoCl 2模拟缺氧条件下与IL-19和IL-20孵育，以使用特异性引物的实时定量PCR分析促炎细胞因子表达模式。DLS患者小关节内IL-19和IL-20呈阳性染色，并伴有TNF-α、IL-1β和MCP-1的丰富表达。IL-19和IL-20受体（IL-20 R1和IL-20 R2）在DLS患者的小关节和黄韧带组织中的软骨细胞和纤维细胞/成纤维细胞上表达。IL-20和IL-1β在关节突关节中的表达有显著相关性。在体外实验中，IL-19和IL-20在CoCl 2模拟缺氧条件下上调原代培养的DLS椎间盘细胞中IL-1β、IL-6、TNF-α、IL-8、VEGF和MCP-1的表达。在DLS患者中，IL-19、IL-20及其受体以及促炎细胞因子（TNF-α、IL-1β和MCP-1）在小关节中的表达高于其他组织;因此，炎症的病因可能更以小关节为中心。IL-19和IL-20诱导椎间盘细胞表达促炎细胞因子，可能在DLS的发病机制中发挥作用。
Degenerative lumbar spondylolisthesis (DLS) is a major cause of spinal canal stenosis and is often related to lower back pain. IL-20 is emerging as a potent angiogenic, chemotactic, and proinflammatory cytokine related to several chronic inflammatory bone disorders likes intervertebral disc herniation, rheumatoid arthritis (RA), osteoporosis, and bone fracture. IL-19 also acts as a proinflammatory cytokine in RA. The aim of the present study was to investigate whether IL-19 and IL-20 are involved in DLS and compare three different tissues including disc, facet joint, and ligamentum flavum of patients with DLS to verify which tissue is affected more by inflammation. Disc, facet joint and ligamentum flavum from 13 patients with DLS was retrieved, and the expression pattern of IL-19, IL-20, IL-20R1, IL-20R2, TNF-α, IL-1β, and MCP-1 was evaluated using immunohistochemical staining with specific antibodies. The disc cells were isolated and incubated with IL-19 and IL-20 under CoCl2-mimicked hypoxic conditions to analyze the proinflammatory cytokine expression pattern using real-time quantitative PCR with specific primers. IL-19 and IL-20 were positively stained and accompanied by abundant expression of TNF-α, IL-1β, and MCP-1 in facet joints of DLS patients. IL-19 and IL-20’s receptors (IL-20R1 and IL-20R2) were expressed on chondrocytes and fibrocytes/fibroblasts in facet joint and ligamentum flavum tissues from patients with DLS. There was a significant correlation between the expression of IL-20 and IL-1β in facet joint. In vitro assay, IL-19 and IL-20 upregulated the expression of IL-1β, IL-6, TNF-α, IL-8, VEGF, and MCP-1 in primary cultured DLS disc cells under CoCl2-mimicked hypoxic conditions. IL-19, IL-20, and their receptors as well as proinflammatory cytokines (TNF-α, IL-1β, and MCP-1) were expressed more in facet joints than the other tissues in patients with DLS; therefore, the etiology of inflammation might be more facet-centric. IL-19 and IL-20 induced proinflammatory cytokine expression in disc cells and might play a role in the pathogenesis of DLS.
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DOI：
10.1016/j.bbrc.2017.05.086
发表时间：
2017-07-01
影响因子：
3.1
作者：
Liu, Zhuochao;Li, Changwei;Zhang, Xingkai
通讯作者：
Zhang, Xingkai
影响因子：
5
作者：
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影响因子：
3
作者：
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Urban, JPG
影响因子：
3
作者：
DEYO, RA;TSUIWU, YJ
通讯作者：
TSUIWU, YJ
影响因子：
1.7
作者：
Igarashi, Akira;Kikuchi, Shin-ichi;Konno, Shin-ichi
通讯作者：
Konno, Shin-ichi