Optimum Culture Conditions for Production by Pseudomonas chlororaphis B23 of Nitrile Hydratase
Optimum Culture Conditions for Production by Pseudomonas chlororaphis B23 of Nitrile Hydratase
复制标题
绿针假单胞菌B23生产腈水合酶的最佳培养条件
DOI：
10.1271/bbb1961.50.2859
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
I. Watanabe
中科院分区：
文献类型：
--
作者：
H. Yamada;K. Ryuno;Torn Nagasawa;Kanehiko Enomoto;I. Watanabe
我们寻求绿针假单胞菌B23生产腈水合酶活性的最佳培养条件。此外，铁和亚铁离子和使用甲基丙烯酰胺作为诱导剂大大增强腈水合酶的形成。当绿针假单胞菌B23在25 ℃下在由1g蔗糖、0.5g甲基丙烯酰胺、0.2g L-半胱氨酸、0.2g L-谷氨酸（Na）、0.2g L-脯氨酸、50 mg KH 2 PO 4、50 mg K2 HPO 4、50 mg MgSO 4·7 H2O、当pH值控制在7.5 ~ 7.8时，每100 ml自来水中加入1 mg的FeSO 4·7 H20，发酵液的酶活是文献报道的900倍。
We sought optimum culture conditions for the production by Pseudomonas chlororaphis B23 of nitrile hydratase activity. Addition of ferric and ferrous ions and the use of methacrylamide as an inducer greatly enhanced nitrile hydratase formation. When P. chlororaphis B23 was cultivated for 26 hr at 25°C in a medium consisting of 1 g of sucrose, 0.5 g of methacrylamide, 0.2 g of l-cysteine, 0.2 g of l-glutamate (Na), 0.2g of l-proline, 50 mg of KH2PO4, 50 mg of K2HPO4, 50 mg of MgSO4·7H20, and 1 mg of FeSO4·7H20 per 100 ml of tap water with the pH controlled at pH 7.5 to 7.8, the enzyme activity in the culture broth was 900-times that previously reported.