Measurement of human immunodeficiency virus type 1 plasma virus load based on reverse transcriptase (RT) activity:: Evidence of variabilities in levels of virion-associated RT
Measurement of human immunodeficiency virus type 1 plasma virus load based on reverse transcriptase (RT) activity:: Evidence of variabilities in levels of virion-associated RT
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DOI：
10.1086/515272
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发表时间：
1998-05-01
影响因子：
6.4
通讯作者：
Heneine, W
中科院分区：
文献类型：
--
作者：
Lerma, JGG;Yamamoto, S;Heneine, W
基于功能性逆转录酶（RT）水平的病毒载量通过使用Amp-RT（一种超灵敏的RT测定法）在50例人类免疫缺陷病毒（HIV） 1型感染者、10例HIV-1血清转化组的87例样本和100例未感染者的血浆中进行了测量。在50例临床样本中，38例（76%）Amp-RT阳性，而所有未感染对照均为阴性。RNA与RT水平的Pearson相关系数为。所有样品73。86的血清转换样本，和。49用于临床样本。计算出的RT活性与病毒粒子RNA的比值在感染的早期和晚期变化很大。8个血清转化组和35个个体临床样本中的12个（34.3%）的平均RT:RNA比率显著低于参考病毒的比率。然而，随着时间的推移，个体血清转换的比率是稳定的。这些数据表明，RT活性可用于定量血浆病毒载量，并为hiv -1感染者中不同水平的病毒相关RT提供证据。
Virus load based on levels of functional reverse transcriptase (RT) was measured in plasma from 50 human immunodeficiency virus (HIV) type 1-infected persons, in 87 samples from 10 HIV-1 seroconversion panels, and in 100 uninfected persons by use of Amp-RT, an ultrasensitive RT assay, Of the 50 clinical samples, 38 (76%) were Amp-RT positive, while all uninfected controls were negative. Pearson's correlation coefficient of RNA and RT levels was .73 for all samples, .86 for seroconversion samples, and .49 for clinical samples. Calculated ratios of RT activity to virion RNA varied widely during both early and late stages of infection. Mean RT:RNA ratios in 8 seroconversion panels and in 12 (34.3%) of 35 individual clinical samples were significantly lower than the ratio for a reference virus. However, ratios were stable in individual seroconversions over time. These data demonstrate that RT activity can be used to quantitate plasma virus load and provide evidence of different levels of virion-associated RT among HIV-1-infected persons.