A Resveratrol and Polyphenol Preparation Suppresses Oxidative and Inflammatory Stress Response to a High-Fat, High-Carbohydrate Meal
A Resveratrol and Polyphenol Preparation Suppresses Oxidative and Inflammatory Stress Response to a High-Fat, High-Carbohydrate Meal
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DOI：
10.1210/jc.2010-1812
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发表时间：
2011-05-01
影响因子：
5.8
通讯作者：
Dandona, Paresh
中科院分区：
文献类型：
--
作者：
Ghanim, Husam;Sia, Chang Ling;Dandona, Paresh
背景资料：已知高脂肪、高碳水化合物（HFHC）膳食诱导氧化和炎症应激、血浆内毒素浓度增加以及细胞因子信号传导抑制因子-3（SOCS-3）表达增加。摄入含有白藜芦醇和圆叶葡萄多酚的营养补充剂减少HFHC膳食诱导的氧化和炎症应激并刺激抗氧化转录因子的活性，NF-E2相关因子-2（Nrf-2）及其下游靶点。方法：10名正常健康受试者给予930千卡HFHC餐，含安慰剂或补充剂。氧化应激、炎症、Nrf-2结合活性、内毒素浓度等指标检测Toll样受体4（TLR-4）、CD 14、IL-1 β、TNF α、SOCS-3、Keap-1、NAD（P）H：醌氧化还原酶-1（NQO-1）和GST-P1的表达。补充剂的摄入抑制了膳食诱导的血浆内毒素和LBP浓度升高，p47（phox）、TLR-4、CD 14、SOCS-3、IL-1 β和Keap-1的表达，结论：含有白藜芦醇和圆叶葡萄多酚的补充剂抑制HFHC餐后单核细胞中氧化应激、脂多糖和LBP浓度以及TLR-4、CD 14、IL-1 β和SOCS-3表达的增加。它还刺激特定的Nrf-2活性并诱导相关抗氧化基因NQO-1和GST-P1的表达。这些结果表明，急性抗氧化剂和白藜芦醇和多酚类化合物在人类餐后状态下的抗氧化作用。（临床内分泌代谢杂志96：1409-1414，2011）
Background: High-fat, high-carbohydrate (HFHC) meals are known to induce oxidative and inflammatory stress, an increase in plasma endotoxin concentrations, and an increase in the expression of suppressor of cytokine signaling-3 (SOCS-3).Hypothesis: The intake of a nutritional supplement containing resveratrol and muscadine grape polyphenols reduces HFHC meal-induced oxidative and inflammatory stress and stimulates the activity of the antioxidant transcription factor, NF-E2-related factor-2 (Nrf-2), and its downstream targets.Methods: Ten normal, healthy subjects were given a 930-kcal HFHC meal either with placebo or with the supplement. Indices of oxidative stress, inflammation, Nrf-2 binding activity, the concentrations of endotoxin (lipopolysaccharide) and lipoprotein binding protein (LBP), and the expression of toll-like receptor 4 (TLR-4), CD14, IL-1 beta, TNF alpha, SOCS-3, Keap-1, NAD(P)H:quinone oxidoreductase-1 (NQO-1), and GST-P1 were measured.Results: The intake of the supplement suppressed the meal-induced elevations of plasma endotoxin and LBP concentrations, the expression of p47(phox), TLR-4, CD14, SOCS-3, IL-1 beta, and Keap-1, while enhancing Nrf-2 binding activity and the expression of NQO-1 and GST-P1 genes.Conclusion: A supplement containing resveratrol and muscadine polyphenols suppresses the increase in oxidative stress, lipopolysaccharide and LBP concentrations, and expression of TLR-4, CD14, IL-1 beta and SOCS-3 in mononuclear cells after an HFHC meal. It also stimulates specific Nrf-2 activity and induces the expression of the related antioxidant genes, NQO-1 and GST-P1. These results demonstrate the acute antioxidant and antiinflammatory effects of resveratrol and polyphenolic compounds in humans in the postprandial state. (J Clin Endocrinol Metab 96: 1409-1414, 2011)