Fluorescence quenching in model membranes. 2. Determination of local lipid environment of the calcium adenosinetriphosphatase from sarcoplasmic reticulum.
Fluorescence quenching in model membranes. 2. Determination of local lipid environment of the calcium adenosinetriphosphatase from sarcoplasmic reticulum.
复制标题
模型膜中的荧光猝灭。
DOI：
10.1021/bi00510a033
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Feigenson,GW
中科院分区：
文献类型：
--
作者：
London,E;Feigenson,GW
Erwin London 1和GeraldW. Feigenson* 摘要：荧光淬灭自旋标记的β-磷脂被用来确定不同的β-磷脂物种的亲和力的内在膜蛋白，钙离子-ATP酶的内质网。与Ca 2 + ATP酶接触的磷脂在重组系统中进行检查，在该系统中，酶被掺入到定义的磷脂组合物的模型膜中。蛋白质的局部磷脂环境被认为在每个磷脂结合位点由平衡控制：脂质A+（脂质B-蛋白）^脂质B+（脂质A-蛋白）。
Erwin London1 and GeraldW. Feigenson* abstract: Fluorescence quenching by spin-labeled phos-pholipid is used to determine the affinities of different phos-pholipid species to an intrinsic membrane protein, the Ca2+-ATPase of sacroplasmic reticulum. The phospholipids in contact with the Ca2+ ATPase are examined in a reconstituted sysem in which the enzyme is incorporated into a model membrane of defined phospholipid composition. The local phospholipid environment of the protein is considered to be governed at each phospholipid binding site by an equilibrium: lipid A+(lipid B-protein)^ lipid B+(lipid A-protein).