Radioprotection of hematopoietic tissue by fibroblast growth factors in fractionated radiation experiments
Radioprotection of hematopoietic tissue by fibroblast growth factors in fractionated radiation experiments
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DOI：
10.3109/02841869709001273
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发表时间：
1997-01-01
期刊：
影响因子：
3.1
通讯作者：
Okunieff, P
中科院分区：
文献类型：
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作者：
Ding, I;Huang, KD;Okunieff, P
酸性和碱性成纤维细胞生长因子（FGF（1/2））骨髓保护小鼠在单剂量全身照射（TBI）实验中的剂量修正因子（DMF）约为1.15。CFU-C分析表明，机制之一是辐射剂量反应曲线的肩部增加，因此，分次治疗的保护作用可能更大。在0 h、8 h、24 h和32 h时将四等份TBI递送至C3 H/He小鼠。FGF（1/2）剂量为在第一次放射剂量前24和4小时静脉注射3 μ g。FGF（2）处理的小鼠比盐水处理的小鼠具有显著的存活优势，DMF为1.22 +/- 0.07（p < 0.01）。添加第三剂量的FGF（2）对LD 50/30（在第30天测量的对50%动物致死的辐射剂量）没有额外的益处（DMF = 1.23 +/- 0.06，p < 0.01）。FGF（1）在分级分离时不那么有效（DMF = 1.04 +/- 0.03）。FGF 2处理的小鼠中存活率增加是由于骨髓造血细胞和外周WBC、RBC和血小板的更快恢复。FGF（2）可能被证明是一种有用的治疗反应调节剂在临床分割照射。
Acidic and basic fibroblast growth factors (FGF(1/2)) myeloprotect mice in single dose total body irradiation (TBI) experiments with a dose modification factor (DMF) of approximate to 1.15. CFU-C assay suggests that one of the mechanisms is augmentation of the shoulder of the radiation dose response curve, and thus protection could be greater with fractionation. Four equal fractions of TBI were delivered to C3H/He mice at times 0 h, 8 h, 24 h, and 32 h. FGF(1/2) dose was 3 mu g per iv injection given 24 and 4 hrs before the first radiation dose. FGF(2) treated mice had a significant survival advantage over saline-treated mice with a DMF of 1.22 +/- 0.07 (p < 0.01). Adding a third dose of FGF(2), had no additional benefit on LD50/30 (dose of radiation lethal to 50% of animals measured at day 30) (DMF = 1.23 +/- 0.06, p < 0.01). FGF(1) was not as effective with fractionation (DMF = 1.04 +/- 0.03). Increased survival in FGF2 treated mice was due to the a more rapid recovery of bone marrow hematopoietic cells and peripheral WBC, RBC and platelets. FGF(2) may prove a useful treatment response modifier in clinical fractionated irradiation.