Kinetics and mechanism of benzoylformate decarboxylase using 13C and solvent deuterium isotope effects on benzoylformate and benzoylformate analogues.
Kinetics and mechanism of benzoylformate decarboxylase using 13C and solvent deuterium isotope effects on benzoylformate and benzoylformate analogues.
复制标题
使用 13C 和溶剂氘同位素对苯甲酰甲酸和苯甲酰甲酸类似物的苯甲酰甲酸脱羧酶的动力学和机制。
DOI：
10.1021/bi00406a058
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Cook,PF
中科院分区：
文献类型：
--
作者：
Weiss,PM;Garcia,GA;Kenyon,GL;Cleland,WW;Cook,PF
材料和方法总则。苯甲酰甲酸、硫胺素焦磷酸、牛血清白蛋白、苯丙酮酸、反式肉桂酸、苯甲酸、苯氧基乙酸、马肝醇de1缩写：CHES，2-(7V-环己氨基)乙磺酸；HEPES，.，V-(2-羟乙基)哌嗪-JV‘-2-乙磺酸；TAPS，3-[三(羟甲基)甲基]氨基]丙磺酸；MES，2-(JV-吗啉)乙磺酸；BFD，苯甲酰基十二酸酶；BF，苯甲酰甲酸酯；TPP，焦磷酸硫胺；BSA，牛血清白蛋白；H，肝醇脱氢酶；氢APADH，3-乙酰吡啶腺嘌呤二核苷酸(简式)；十二烷基硫酸钠-聚丙烯酰胺凝胶电泳；熔点；ppm，百万分之几。
Materials and MethodsGeneral. Benzoylformic acid, thiamine pyrophosphate, bovine serum albumin, phenylpyruvate, trans-cinnamic acid, benzoic acid, phenoxyacetic acid, equine liver alcohol de-1 Abbreviations: CHES, 2-(7V-cyclohexylamino) ethanesulfonic acid; HEPES,., V-(2-hydroxyethyl) piperazine-jV'-2-ethanesulfonic acid; TAPS, 3-[[tris (hydroxymethyl) methyl] amino] propanesulfonic acid; MES, 2-(jV-morpholino) ethanesulfonic acid; BFD, benzoylformate decarboxylase; BF, benzoylformate; TPP, thiamine pyrophosphate; BSA, bovine serum albumin; LADH, equine liver alcohol dehydrogenase; APADH, 3-acetylpyridine adenine dinucleotide (reduced form); SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; mp, melting point; ppm, parts per million.