Resveratrol prevents development of eosinophilic rhinosinusitis with nasal polyps in a mouse model
Resveratrol prevents development of eosinophilic rhinosinusitis with nasal polyps in a mouse model
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DOI：
10.1111/all.12132
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发表时间：
2013-07-01
期刊：
影响因子：
12.4
通讯作者：
Min, Y. -G.
中科院分区：
文献类型：
--
作者：
Kim, S. -W.;Kim, D. W.;Min, Y. -G.
研究背景近年来，嗜酸性粒细胞性慢性鼻窦炎伴鼻息肉（CRSwNP）小鼠模型的建立，为开发治疗或预防嗜酸性粒细胞性CRSwNP的新药及阐明其发病机制提供了可能。我们研究了白藜芦醇对CRSwNP的治疗效果及其作用机制，使用小鼠model.Methodsafter诱导嗜酸性CRSwNP，白藜芦醇的治疗效果进行了测试和比较与曲安奈德。使用苏木精和伊红评价组织学变化，使用天狼星红评价嗜酸性粒细胞，使用Masson三色染色评价胶原蛋白。通过实时定量PCR评估白细胞介素（IL）-4，IL-5，前列腺素D合酶和白三烯C-4合酶基因的表达水平。免疫组化染色和Western blot analysis.ResultsThe程度的嗜酸性粒细胞浸润和上皮下纤维化的环氧合酶-2，5-lipoxygense水平进行了评价，显着降低了高剂量的白藜芦醇，其效力是类似的曲安奈德。IL-4，IL-5，前列腺素D合成酶，和白三烯C-4合成酶基因的表达水平显着降低低剂量或高剂量白藜芦醇的管理。5-脂氧合酶的产生强烈抑制高剂量resveratrol.ConclusionsResveratrol可能是有用的嗜酸性粒细胞CRSwNP的预防。其作用的一个关键机制被认为是其抗炎作用，特别是对嗜酸性粒细胞，通过抑制脂氧合酶途径。
BackgroundSince the recent establishment of a murine model of eosinophilic chronic rhinosinusitis with nasal polyps (CRSwNP), both the development of new drugs for treatment or prevention of eosinophilic CRSwNP and elucidation of their pathogenesis have been feasible. We investigated the therapeutic effects of resveratrol on CRSwNP and its mechanism of action using a murine model.MethodsAfter induction of eosinophilic CRSwNP, the therapeutic effects of resveratrol were tested and compared with those of triamcinolone acetonide. Histopathologic changes were evaluated using hematoxylin and eosin for overall inflammation, Sirius red for eosinophils, and Masson's trichrome stain for collagen. The expression levels of the interleukin (IL)-4, IL-5, prostaglandin D synthase, and leukotriene C-4 synthase genes were assessed by quantitative real-time PCR. Cyclooxygense-2 and 5-lipoxygense levels were evaluated by immunohistochemical staining and Western blot analysis.ResultsThe degree of eosinophilic infiltration and subepithelial fibrosis was significantly decreased by administration of high-dose resveratrol, the potency of which was similar to that of triamcinolone acetonide. The expression levels of the IL-4, IL-5, prostaglandin D synthase, and leukotriene C-4 synthase genes were significantly decreased by administration of low- or high-dose resveratrol. The production of 5-lipoxygenase was strongly inhibited by high-dose resveratrol.ConclusionsResveratrol may be useful for the prevention of eosinophilic CRSwNP. A key mechanism of its action is believed to be its anti-inflammatory effect, particularly on eosinophils, by inhibiting the lipoxygenase pathway.