Electroacupuncture improves glucose tolerance through cholinergic nerve and nitric oxide synthase effects in rats
Electroacupuncture improves glucose tolerance through cholinergic nerve and nitric oxide synthase effects in rats
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DOI：
10.1016/j.neulet.2011.02.071
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发表时间：
2011-04-25
影响因子：
2.5
通讯作者：
Chang, Shih-Liang
中科院分区：
文献类型：
--
作者：
Lin, Rong-Tsung;Chen, Ching-Yuan;Chang, Shih-Liang
本研究旨在探讨电针双侧足三里穴对正常大鼠糖耐量的影响及其机制。采用静脉葡萄糖耐量试验（IVGTT）观察电针对大鼠葡萄糖耐量的影响。电针组在ST-36处进行电针，设置为15 Hz，10 mA，60 min;对照组进行相同的治疗，但不进行电针。分别单独或同时注射阿托品、半胆碱-3（HC-3）或NG-硝基-L-精氨酸甲酯（L-NAME），并进行电针治疗，以研究与对照组相比血糖水平的差异。采集血浆样品用于测定血浆葡萄糖和游离脂肪酸（FFA）水平。Western blot检测胰岛素信号蛋白和nNOS的表达，探讨电针与糖耐量改善的相关性。与对照组相比，EA组的血糖水平显著降低。单独给予L-NAME、阿托品或HC-3治疗后，EA组和对照组之间的血糖水平差异显著，但L-NAME和阿托品或L-NAME和HC-3联合治疗的血糖水平无显著差异。电针可降低IVGTT时骨骼肌FFA水平，增强胰岛素信号蛋白（IRS 1）和nNOS活性。总之，EA刺激胆碱能神经和一氧化氮合酶，降低血浆FFA水平，改善糖耐量。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
The purpose of this investigation was to evaluate the effect and mechanisms of electroacupuncture (EA) at the bilateral Zusanli acupoints (ST-36) on glucose tolerance in normal rats. Intravenous glucose tolerance test (IVGTT) was performed to examine the effects of electroacupuncture (EA) on glucose tolerance in rats. The EA group underwent EA at the ST-36, with settings of 15 Hz, 10 mA, and 60 min: the control group underwent the same treatments, but without EA. Atropine, hemicholinium-3 (HC-3) or NG-nitro-L-arginine methyl ester (L-NAME) were injected into the rats alone or simultaneously and EA was performed to investigate differences in plasma glucose levels compared to the control group. Plasma samples were obtained for assaying plasma glucose and free fatty acid (FFA) levels. Western blot was done to determine the insulin signal protein and nNOS to exam the correlation between EA and improvement in glucose tolerance. The EA group had significantly lower plasma glucose levels compared to the control group. Plasma glucose levels differed significantly between the EA and control groups after the administration of L-NAME, atropine, or HC-3 treatments alone, but there were no significant differences in plasma glucose with combined treatment of L-NAME and atropine or L-NAME and HC-3. EA decreased FFA levels and enhanced insulin signal protein (IRS1) and nNOS activities in skeletal muscle during IVGTT. In summary, EA stimulated cholinergic nerves and nitric oxide synthase for lowering plasma FFA levels to improve glucose tolerance. (C) 2011 Elsevier Ireland Ltd. All rights reserved.